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Anti-TRIP8b (constant) [N212/7R]

Plasmid

#177514

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (constant) (Rat). Derived from hybridoma N212/7.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (constant) (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S263E

Plasmid

#141340

Purpose

Lentiviral vector for expression of human NHEJ1-S263E in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S263E (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S263E

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S263A

Plasmid

#141341

Purpose

Lentiviral vector for expression of human NHEJ1-S263A in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S263A (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S263A

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pQC V5 mKO2 HuR.DelHNS IRES Puro

Plasmid

#110389

Purpose

γ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Retroviral

Tags

V5 and mKO2

Expression

Mammalian

Mutation

HNS (HuR nuclear-cytoplasmic shuttling sequence) …

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b [N212/7R]

Plasmid

#164154

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (Rat). Derived from hybridoma N212/7.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Iduna/RNF146 [N201/35R]

Plasmid

#164157

Purpose

Mammalian Expression Plasmid of anti-Iduna/RNF146 (Mouse). Derived from hybridoma N201/35.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Iduna/RNF146 (Mus musculus) recombinant mouse monoclonal antibody (Rnf146 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-ZNF423 [N328B/37R]

Plasmid

#140077

Purpose

Mammalian Expression Plasmid of anti-ZNF423 (Human). Derived from hybridoma N328B/37.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-ZNF423 (Human) recombinant mouse monoclonal antibody (ZNF423 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-mRFP

Plasmid

#117787

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-REEP1/2 [N326D/29R]

Plasmid

#114510

Purpose

Mammalian Expression Plasmid of anti-REEP1/2 (Mouse). Derived from hybridoma N326D/29.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-REEP1/2 (Mus musculus) recombinant mouse monoclonal antibody (Reep1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-PARIS/ZNF746 [N196/16R]

Plasmid

#114475

Purpose

Mammalian Expression Plasmid of anti-PARIS/ZNF746 (Human). Derived from hybridoma N196/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PARIS/ZNF746 (Homo sapiens) recombinant mouse monoclonal antibody (ZNF746 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

Plasmid

#120295

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Malin [N85/18.1R]

Plasmid

#114513

Purpose

Mammalian Expression Plasmid of anti-Malin (Human). Derived from hybridoma N85/18.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Malin (Homo sapiens) recombinant mouse monoclonal antibody (NHLRC1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Stonin-2 [N346/9R]

Plasmid

#114484

Purpose

Mammalian Expression Plasmid of anti-Stonin-2 (Human). Derived from hybridoma N346/9.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Stonin-2 (Homo sapiens) recombinant mouse monoclonal antibody (STON2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-GFP-PGK-Puro

Plasmid

#110862

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-GFP-PGK-Puro

Plasmid

#110863

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shHRASLS.2

Plasmid

#110324

Purpose

TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

HRASLS (PLAAT1 Homo sapiens, Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shHRASLS.1

Plasmid

#110323

Purpose

TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

HRASLS (PLAAT1 Homo sapiens, Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1893-1

Plasmid

#105248

Purpose

human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

Insertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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