We narrowed to 13,031 results for: Hal
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Plasmid#86263PurposeDonor vector for 3' FLAG tag of human MBD1_iso1DepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only
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actc1b-mKate2-EHD1b
Plasmid#109514PurposeMuscle specific zebrafish expression of mKate2 fused to EHD1bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
KY901: pMVP (L3-L2) P2A-mCherry + polyA
Plasmid#121766PurposepMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.DepositorInsertP2A-mCherry + polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168169PurposeExpression of YFP-BeCyclOp(A-2x) in BWMs of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
TagsYFPExpressionWormAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
LC402: pMVP (L3-L2) eGFP + polyA
Plasmid#121763PurposepMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.DepositorInserteGFP + polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG802: pMVP (L3-L2) V5 epitope tag + polyA
Plasmid#121755PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertV5 epitope tag + polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-LactC2
Plasmid#109499PurposeMuscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)DepositorInsertactc1b-mKate2-LactC2
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWL502-PAM
Plasmid#174382PurposePlasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterraneiDepositorInsertPAM-spacer
UseCRISPR; Archaeal expressionExpressionBacterialAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP472
Plasmid#134997PurposeInterspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertInters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP498
Plasmid#134998PurposeClustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertClust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP650
Plasmid#135000PurposeVP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoRDepositorInsertpUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
UseLentiviral and Synthetic BiologyTagsDpnI(aa146-254), V5 tag, and VP-64ExpressionMammalianMutationDpnI truncated to aa146-254Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7a
Plasmid#109655PurposeMuscle specific zebrafish expression of mKate2 fused to rab7aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7b
Plasmid#109656PurposeMuscle specific zebrafish expression of mKate2 fused to rab7bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS
Plasmid#121821PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9 (open)
UseGateway Cloning and Synthetic BiologyTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab8a
Plasmid#109657PurposeMuscle specific zebrafish expression of mKate2 fused to rab8aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
KW701: pMVP (L5-L4) AID
Plasmid#121723PurposepMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.DepositorInsertAID (open)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA501: pMVP (L3-L2) pA; CMV::TETa-pA
Plasmid#121801PurposepMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.DepositorInsertpolyA + CMV::TETa-polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA201: pMVP (L3-L2) P2A-eCFP + WPRE
Plasmid#121776PurposepMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.DepositorInsertP2A-eCFP + WPRE
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
II901: pMVP (L5-L4) FKBP DD
Plasmid#121720PurposepMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.DepositorInsertFKBP Degron Domain (open)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only