We narrowed to 6,944 results for: crispr cas9 plasmids
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Plasmid#204312PurposeFor generating targeting inversions. C-terminal split GFP, expressed in eyes.DepositorInsert3XP3-GFPc
UseCRISPRPromoter3XP3Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT22
Plasmid#204311PurposeFor generating targeting inversions. N-terminal split GFP, expressed in eyes.DepositorInsert3XP3-GFPn
UseCRISPRPromoter3XP3Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
Tags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianPromoterCMVAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS285
Plasmid#73734PurposeSapTrap egl-13 NLS connector donor plasmidDepositorInsertegl-13 NLS
UseCRISPRAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
KO plasmid
Plasmid#135970PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SpRYgest_targetplasmid (KAC833)
Plasmid#181748PurposepUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.DepositorTypeEmpty backboneUseSubstrate plasmidAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-dSa-VP64
Plasmid#99680PurposeExpresses dSa Cas9 fused to gold standard VP64 activatorDepositorArticleInsertdCas9
UseAAVTagsVP64Mutationdead Cas9Available SinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSHS293
Plasmid#87348PurposeD435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/D435C/C574S/E945CPromoterT7Available SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only