We narrowed to 18,626 results for: Met
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Plasmid#145878PurposeInsect Expression of DmHPat_500-968DepositorInsertDmHPat_500-968 (Patr-1 Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA
Plasmid#170767PurposeMethylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coliDepositorInsertsM.XbaI
M.EcoRI
M.SalI
ExpressionBacterialPromoterrhamnoseAvailable SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
F0TBY7
Plasmid#163245PurposeInducible expression of UniProt identifier F0TBY7DepositorInsertF0TBY7
Tags10xHis and T7ExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAAAG
Plasmid#170096PurposedeGFP reporter plasmid with CAAAG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAATG
Plasmid#170097PurposedeGFP reporter plasmid with CAATG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTAAT
Plasmid#170098PurposedeGFP reporter plasmid with GTAAT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTATT
Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PacI
Plasmid#170100PurposedeGFP reporter plasmid with unique PacI restriction enzyme recognition siteDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyPromoterJ23119Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas5
Plasmid#170090Purposeexpresses E. coli type I-E Cas5DepositorInsertE. coli type I-E Cas5
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas7
Plasmid#170092Purposeexpresses E. coli type I-E Cas7DepositorInsertE. coli type I-E Cas7
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas8
Plasmid#170093Purposeexpresses E. coli type I-E Cas8DepositorInsertE. coli type I-E Cas8
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCse2
Plasmid#170094Purposeexpresses E. coli type I-E Cse2DepositorInsertE. coli type I-E Cse2
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm No carrier NikJC199U
Plasmid#174361PurposeExpression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Tags8xHis and TEVExpressionBacterialMutationC199UPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E08: attB2r-mTFP1-attB3
Plasmid#162904PurposeGateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteinsDepositorInsertmonomeric Teal fluorescent protein
UseSynthetic BiologyAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH010
Plasmid#157645PurposesMHT-113 fused non-dimerizing GRX2-C37A mutantDepositorInsertssMHT(1-113)-GRX2_C37A
GRX2_C37A-sMHT(114-230)
ExpressionBacterialAvailable SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRNA2
Plasmid#174005PurposeUsed to generate stable mRNA for heterologous expression experiments (no polyA)DepositorInserteGFP
ExpressionMammalianPromoterCMVAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+AtAct2t
Plasmid#170882PurposePhytobrick - AtACT2tDepositorInsertAtACT2t
ExpressionPlantMutation426C>T to remove BbsI restriction siteAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+NOSt
Plasmid#170885PurposePhytobrick - NOStDepositorInsertNOSt
ExpressionPlantMutation300C>T to remove BbsI restriction siteAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD+35St
Plasmid#170886PurposePhytobrick - 35StDepositorInsert35St
ExpressionPlantAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E26: attB4-T7p>-attB5
Plasmid#162982PurposeGateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoterDepositorInsertbacteriophage T7 promoter
UseSynthetic BiologyAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C511-E15: attB5r-ffluc2-nostop-attB1r
Plasmid#162939PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteinsDepositorInsertFirefly luciferase
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E40: attB4-EFSp>-attB1r
Plasmid#162928PurposeGateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoterDepositorInsertshort form of human EF1 alpha promoter
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E34: attB4-mCMVp>-attB1r
Plasmid#162926PurposeGateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoterDepositorInsertminimal CMV IE1 promoter
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C238-E07: attB2r-SV40polyA-attB3
Plasmid#162917PurposeGateway attB2r/attB3 entry clone containing a polyA sequence for transcription terminationDepositorInsertSV40 polyadenylation sequence
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E24: attB2r-Strep2-attB3
Plasmid#162913PurposeGateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteinsDepositorInsertStrep2 epitope tag
UseSynthetic BiologyAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C231-E02: attB2r-NanoLuc-attB3
Plasmid#162901PurposeGateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteinsDepositorInsertNanoluciferase
UseSynthetic BiologyAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E06: attB2r-mCherry-attB3
Plasmid#162903PurposeGateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteinsDepositorInsertmonomeric Cherry fluorescent protein
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E04: attB4-CMV51p>-attB5
Plasmid#162973PurposeGateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoterDepositorInsertoptimized human CMV promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E17: attB4-PGKp>-attB5
Plasmid#162976PurposeGateway attB4/attB5 entry clone for mammalian expression using the human PGK promoterDepositorInserthuman pGK promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-FRB1C_no-linker
Plasmid#162434Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertLyn-CFP-FRB1C_no_linker
ExpressionMammalianPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E36: attB4-CMV51p>-attB1r
Plasmid#162927PurposeGateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoterDepositorInsertoptimized CMV promoter
UseSynthetic BiologyAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E25: attB2r-mVenus-attB3
Plasmid#162905PurposeGateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteinsDepositorInsertmonomeric Venus fluorescent protein
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E34: attB2r-iRFP670-attB3
Plasmid#162908PurposeGateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E11: attB5r-mTFP1-nostop-attB1r
Plasmid#162942PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteinsDepositorInsertmonomeric Teal fluorescent protein
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E19: attB4-mPol2p>-attB1r
Plasmid#162971PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E29: attB5r-mKate2-nostop-attB1r
Plasmid#162946PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteinsDepositorInsertmonomeric Kate2 fluorescent protein
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E15: attB5r-mVenus-nostop-attB1r
Plasmid#162943PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteinsDepositorInsertmonomeric Venus fluorescent protein
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E26: attB4-SV40p>-attB1r
Plasmid#162923PurposeGateway attB4/attB1r entry clone for mammalian expression using the SV40 promoterDepositorInsertSV40 immediate early promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E21: attB4-PGKp>-attB1r
Plasmid#162921PurposeGateway attB4/attB1r entry clone for mammalian expression using the human PGK promoterDepositorInserthuman pGK promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E07: attB2r-HA-attB3
Plasmid#162911PurposeGateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteinsDepositorInsertHA epitope tag
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E06: attB2r-FLAG-attB3
Plasmid#162910PurposeGateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteinsDepositorInsertFLAG epitope tag
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E05: attB2r-V5-attB3
Plasmid#162909PurposeGateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteinsDepositorInsertV5 epitope tag
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID6JY
Plasmid#165049PurposeIntegrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID8Z105
Plasmid#165057PurposeIntegrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID9L105
Plasmid#165055PurposeIntegrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID9L
Plasmid#165052PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5Z
Plasmid#165047PurposeIntegrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only