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pET28b_Q69L

Plasmid

#186272

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His-tagged at N terminal

Expression

Bacterial

Mutation

Changed Q69 into L

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMALp2x-CARP-1

Plasmid

#41727

Depositor

Koji Okamoto

Article

Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

clam ADP-ribosylating protein-1

Use

Bacterial subcloning

Mutation

deleted 5'-UTR nucleotides 1-64

Promoter

pMB1

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pT2Apuro-Stargazin-mEGFP-iLID

Plasmid

#178529

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-L1T3D

Plasmid

#33276

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L1 (L1, L1 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

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etnppl-pGEMtEasy

Plasmid

#192263

Purpose

Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

Depositor

Toshihide Yamashita

Article

Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

Insert

ethanolamine phosphate phospholyase (Etnppl Mouse)

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfCCK-0.5k-Venus-WPRE

Plasmid

#156392

Purpose

Expresses Venus under CCK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSST-0.3k-Venus-WPRE

Plasmid

#156393

Purpose

Expresses Venus under SST gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-SynTetOff-GFP

Plasmid

#190230

Purpose

Gateway entry vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Expression

Bacterial

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Promoter

Endogenous T/brachyury

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHDAdVenus-geo-TK

Plasmid

#26423

Depositor

Kohnosuke Mitani

Article

Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

PGK-b-geo, HSV-TK, CAG-Venus

Use

Adenoviral

Expression

Mammalian

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pUb-tdTomato-LP-Neo-LP

Plasmid

#231509

Purpose

Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

tdTomato fluorescent protein

Use

Unspecified; Acanthamoeba castellanii neff strain

Promoter

Acanthamoeba polyubiquitin

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGAPDH-Cre-HYG

Plasmid

#231510

Purpose

Expresses Cre recombinase in Acanthamoeba

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

Cre recombinase

Use

Unspecified; Acanthamoeba castellanii neff strain

Tags

EGFP and SV40 NLS

Promoter

Acanthamoeba GAPDH

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET30 SUMO-1 G97

Plasmid

#13381

Depositor

Mary Dasso

Article

Saitoh et al Curr Biol. 1998 Jan 15. 8(2):121-4.

Insert

SUMO-1, G97 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

G97 mutant: lacks 4 amino acids from the C-termin…

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfvAChT-1.8k-Venus-WPRE

Plasmid

#156389

Purpose

Expresses Venus under vAChT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-ERas-IP

Plasmid

#13826

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pT2ADW-lox-mCherry-hyBRET-ERK-NLS

Plasmid

#170290

Purpose

For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

lox-mCherry-lox-hyBRET-ERK-NLS

Expression

Mammalian

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2-SYN-GFP-BGHpA

Plasmid

#190229

Purpose

Expresses EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CRY2-mCherry

Plasmid

#178525

Purpose

Expresses CRY2-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CRY2-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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