We narrowed to 10,052 results for: transfer

Showing: 3221 - 3240 of 10052 results

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pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Plasmid

#219781

Purpose

CaST-P2A for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1h

Plasmid

#190473

Purpose

Express the high-affinity histamine sensor GRAB_HA1h in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1h (high affinity)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-VGAT-WPRE-pA

Plasmid

#39320

Depositor

Bernardo Sabatini

Article

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV; R

Available Since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Electra2-P2A-EGFP

Plasmid

#184936

Purpose

Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

Plasmid

#178960

Purpose

Expresses saCas9 protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Staphylococcus aureus Cas9 (SaCas9)

Use

AAV

Tags

3x HA

Promoter

gfaABC1D (astrocyte specific)

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179120

Purpose

Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

control sgRNA

Use

AAV

Promoter

U6

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126216

Purpose

Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA

Plasmid

#109317

Purpose

Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(H134R)-EYFP

Plasmid

#26969

Purpose

AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

Has Service

AAV1, AAV2, AAV5, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Histidine 134 is mutated to Arginine

Promoter

CaMKIIa

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1011-luc2

Plasmid

#227106

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1011

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-2xNLS-mTurquoise2

Plasmid

#104053

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Promoter

GFAP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-GG

Plasmid

#213039

Purpose

AAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)

Depositor

Fredrik Sterky

Article

De La Cruz et al Bio Protoc. 2024 Nov 5;14(21):e5097. doi

Type

Empty backbone

Use

AAV; Vector for golden gate assembly

Tags

N/A

Promoter

N/A

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-tMCK-ER-TurboID

Plasmid

#149412

Purpose

Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

tMCK

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Has Service

AAV6 and AAV8

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CA-FLEX-dtA

Plasmid

#232236

Purpose

Expresses diphtheria toxin A subunit in Cre-positive cells.

Depositor

Naoshige Uchida

Article

Tsutsui-Kimura et al Nat Neurosci. 2025 Apr;28(4):795-810. do

Insert

Diphtheria toxin A subunit

Use

AAV

Expression

Mammalian

Promoter

CA

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1013-luc2

Plasmid

#227107

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1013

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1013 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM4Di-mCherry

Plasmid

#161576

Purpose

Expression of hM4Di-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-sCAG-GluClβ.CreON

Plasmid

#196996

Purpose

Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

Depositor

David Bennett

Article

Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

Insert

GluClβ -YFP

Use

AAV and Cre/Lox

Tags

YFP

Promoter

short CAG

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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