We narrowed to 12,677 results for: sequence
-
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…TagsExpressionBacterialMutationPromoterEscherichia coli lacIN promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pFX-mCherry-Rab5
Plasmid#174455PurposeExpress mCherry with early endosome-targeting sequence in mammalian cellsDepositorInsertmCherry with early endosome-targeting sequence
UseTagssmall GTPase (Rab5)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
WNK2 K207A
Plasmid#24570DepositorInsertWNK2` (WNK2 Human)
UseTagsHis6 and V5ExpressionMammalianMutationK207APromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pR008_APAD
Plasmid#204818PurposeExpression Apobec1-ADAR2dd fusion protein as PIE-RBP controlDepositorInsertrat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
UseTagsHA and Flag and huADAR2ddExpressionMammalianMutationPromoterchicken β-actin promoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP5H
Plasmid#188061PurposePROSS-designed versatile peroxidase 5HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
UseTagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation43 PROSS mutations, described in publicationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMRN1-bio-His
Plasmid#53409PurposeExpresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertMMRN1 (MMRN1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPS22B-Cl
Plasmid#160431PurposeExpresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoterDepositorInsertRPS22B (RPS22B Budding Yeast)
UseTagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to change nucleo…PromoterGAL1 and S. cerevisiae GAL1Available sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
UseTagsFirst 8 codons of COX3ExpressionMutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
BirA*-HA-NES
Plasmid#214473PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and NES (Nuclear Exclusion Sequence)ExpressionMammalianMutationR118GPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SIX1_P2A_Hygro_Barcode
Plasmid#120476PurposeBarcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSIX1 (SIX1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only