We narrowed to 4,333 results for: chm

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TDP-43_NTD1-80_Y4R

Plasmid

#104478

Purpose

bacterial expression of TDP-43 NTD Y4R

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

His

Expression

Bacterial

Mutation

Y4R

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-WT

Plasmid

#91986

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87E

Plasmid

#36059

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87E (SNCA Human)

Expression

Bacterial

Mutation

S87E

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pKIF1C-BioID2-HA

Plasmid

#130980

Purpose

Expression of HA-tagged KIF1C-BioID2

Depositor

Anne Straube

Article

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

KIF1C-BioID2-HA (KIF1C Human)

Tags

BioID2-HA

Expression

Mammalian

Mutation

5 silent mutations that make this construct RNAi …

Promoter

CMV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Dnmt3b6-Poly(A)

Plasmid

#65532

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 6

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

Plasmid

#155194

Purpose

Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated sequence of THBS1 (expresses Amino Acid…

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

Plasmid

#155195

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 276-315

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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SMN1-1

Plasmid

#156022

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SMN1 (SMN1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp2

Plasmid

#72693

Purpose

Doxycyline regulated expression of Mesp2 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 2 (Mesp2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST15.1-hRPN13 (285-386)

Plasmid

#61936

Purpose

Expression of hRPN13 (285-386) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-386) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, stop codon intro…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pDEST27-GST-ATXN1 FL(92Q)-S776D

Plasmid

#21757

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 92 Q(Glutamines) and…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pT7-7 asyn S87A

Plasmid

#36058

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87A (SNCA Human)

Expression

Bacterial

Mutation

S87A

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-dKARD

Plasmid

#114053

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-dKARD (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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DMGDH

Plasmid

#155549

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DMGDH (DMGDH Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DDX56

Plasmid

#155530

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX56 (DDX56 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CIR1

Plasmid

#155452

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CIR1 (CIR1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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BTG4

Plasmid

#155428

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG4 (BTG4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(30Q)-S776D

Plasmid

#21755

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

Plasmid

#113041

Purpose

AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-Cys-Linker-BAF_L58R

Plasmid

#101780

Purpose

Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Tags

His14-MBP-SUMO

Expression

Bacterial

Mutation

L58R mutation

Promoter

T5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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