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pEPOR2KN0038

Plasmid

#117605

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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AP625-1

Plasmid

#70051

Purpose

Expresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

eGFP

Tags

3Xflag::TEV::myc and myc::TEV::3Xflag

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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TAL2343

Plasmid

#36763

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTCH1-TALEN_Right (PTCH1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2421

Plasmid

#36822

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

RARA-TALEN_Right (Rara Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2420

Plasmid

#36821

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

RARA-TALEN_Left (Rara Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2219

Plasmid

#36678

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MUTYH-TALEN_Right (Mutyh Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEPOR2KN0039

Plasmid

#117606

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2376

Plasmid

#36787

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SUZ12-TALEN_Left (Suz12 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
TAL2377

Plasmid

#36788

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SUZ12-TALEN_Right (Suz12 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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unc5bCD4d3+4-bio

Plasmid

#36148

Purpose

EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b (netrin-1a) with rat CD4d3+4-bio tag

Depositor

Gavin Wright

Article

Sollner et al Genome Biol. 2009;10(9):R99. Epub 2009 S

Insert

unc5b (unc5b Zebrafish)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

L2V

Promoter

CMV

Available Since

May 9, 2012

Availability

Academic Institutions and Nonprofits only
BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRRG142-Arrowhead

Plasmid

#99097

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Arrowhead

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

Arrowhead in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2246

Plasmid

#36691

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PHF8-TALEN_Left (Phf8 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pFH71

Plasmid

#128214

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAP1680

Plasmid

#137088

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

tagRFP

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pFH99

Plasmid

#128204

Purpose

classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH100

Plasmid

#128205

Purpose

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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