We narrowed to 5,563 results for: org

1. APEX2-6xHp

   Plasmid

   #214411

   Purpose

   Lipid droplet localization of APEX2-6xHp for electron microscopy

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   6x Spastin aa 43-92 (APEX2 Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-EF1a-mTagRFP-T-CAAX

   Plasmid

   #167973

   Purpose

   AAVS1 targeting repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFP-T-CAAX

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-STIM1-NN_puromycin

   Plasmid

   #65310

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Use

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104096

   Purpose

   Expresses TREAT-ARE reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Use

   Tags

   12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1_GI.1_Q62C-A140C

   Plasmid

   #162581

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glutamine 62 changed to Cysteine and Alanine 140 …

   Promoter

   polyhedring

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TVBB N-term-mNeongreen

   Plasmid

   #169226

   Purpose

   Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-TSC22D3

   Plasmid

   #192916

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   TSC22D3 (TSC22D3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. miniSOG-LaminB1-10

   Plasmid

   #57771

   Purpose

   Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104140

   Purpose

   Expresses TREAT-5'TOP reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1_Cre

    Plasmid

    #201198

    Purpose

    AAV vector for cre expression under EF1 promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    cre
    EF1alpha promoter
    

    Use

    AAV

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    nuclear targeting cre

    Promoter

    N/A and hEF1alpha promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DCX(S306D)-2A-mCherry

    Plasmid

    #118292

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Serine 306 changed to Aspartic Acid

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-DCX(S306A)-2A-mCherry

    Plasmid

    #118291

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    changed Serine 306 to Alanine

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1a-hLMX1A-T2A-copGFP

    Plasmid

    #170444

    Purpose

    Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human LMX1A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pEN_TT 3xFLAG-MAVS

    Plasmid

    #158628

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid

    #185289

    Purpose

    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    iLID::eGFP::FTH1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid

    #198054

    Purpose

    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

    Depositor

    Lacra Bintu

    Article

    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

    Inserts

    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G and pEF

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLC-RFP657-CASP8

    Plasmid

    #75164

    Purpose

    LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    CASP8 sgRNA (CASP8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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