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pJ414 - DANCER-1

Plasmid

#136385

Purpose

Expresses DANCER-1 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-1

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-2376

Plasmid

#138974

Purpose

Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

Plasmid

#130689

Purpose

Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, M1961_E1992del

Promoter

CMV

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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LC8-like

Plasmid

#133115

Purpose

Human LC8-like protein, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

LC8-like (LOC392067 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

Plasmid

#130690

Purpose

Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, G1720_Y1846del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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LI F-G Gent

Plasmid

#104522

Purpose

LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Apramycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pPI467

Plasmid

#113208

Purpose

integrating expression vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Tags

mScarlet

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pPI468

Plasmid

#113207

Purpose

integrating expression vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Tags

mScarlet

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSO9

Plasmid

#106366

Purpose

To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.

Depositor

Jonathan Ewbank

Article

Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

Insert

ttTi4348_ SEC _pvit-6_OspF_3'unc54

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_6367-EYFP

Plasmid

#111080

Purpose

Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin R1ACR_6367

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RlACR_497-EYFP

Plasmid

#111078

Purpose

Anion channelrhodopsin from Rhodomonas lens for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RlACR_497

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-61

Plasmid

#92262

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

NHA

Expression

Insect

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-59

Plasmid

#92261

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Mutation

aa 940-1215

Promoter

Ac5

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 AAAA

Plasmid

#87600

Purpose

Encodes Prp5 AAAA for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

D136A, P137A, L138A, D139A

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 TAG

Plasmid

#87603

Purpose

Encodes Prp5 SAT motif to TAG for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

S446T, T448G

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP2

Plasmid

#87591

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp2

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP2 (PRP2 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335R

Plasmid

#87299

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335R

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335R

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-BUD13

Plasmid

#87547

Purpose

Encodes Y2H Gal4 DNA binding domain for Bud13

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

BUD13 (BUD13 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-SLU7

Plasmid

#87597

Purpose

Encodes Y2H Gal4 DNA binding domain for Slu7

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

SLU7 (SLU7 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-CLF1

Plasmid

#87548

Purpose

Encodes Y2H Gal4 DNA binding domain for Clf1

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

CLF1 (CLF1 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 D450G

Plasmid

#87303

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 D450G

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

D450G

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 P369K

Plasmid

#87302

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 P369K

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

P369K

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 H331Q

Plasmid

#87296

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 H331Q

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

H331Q

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 H331D

Plasmid

#87295

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 H331D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

H331D

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 N295D

Plasmid

#87294

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 N295D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

N295D

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 R294L

Plasmid

#87293

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 R294L

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

R294L

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/D450G

Plasmid

#87287

Purpose

Encodes Hsh155 N295D D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & D450G

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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