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Showing: 6521 - 6560 of 8371 results

  1. pLenti Puro HA-Ubiquitin-KO

    Plasmid
    #236292
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin KO mutant
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Ubiquitin KO (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence containing KO mu…
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236292-plasmid-map-sequence-id-468935

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  2. pLenti Puro HA-Ubiquitin-WT

    Plasmid
    #236289
    Purpose
    Lentiviral vector expressing HA-tagged wild type ubiquitin
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236289-plasmid-map-sequence-id-467836

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  3. pCAGGS-FLAG-BubR1-D911A

    Plasmid
    #200815
    Purpose
    expresses BubR1-D911A in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Change Aspartic acid 911 to Alanine
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200815-plasmid-map-sequence-id-416909

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  4. 1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE

    Plasmid
    #231804
    Purpose
    Label centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.
    Depositor
    Annette Oxenius
    Insert
    Kaede-CETN1 (Cetn1 Mouse)
    Use
    Lentiviral
    Tags
    Kaede
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231804-plasmid-map-sequence-id-460688

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  5. hSyn-eGFP-DDHD2

    Plasmid
    #232463
    Purpose
    Expresses GFP tagged DDHD2 lipase in neurons
    Depositor
    Timothy Ryan
    Insert
    DDHD2 (DDHD2 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232463-plasmid-map-sequence-id-460234

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  6. pCJ1022

    Plasmid
    #228762
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Pkd2 gRNAs (Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    228762-plasmid-map-sequence-id-455850

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  7. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

    Plasmid
    #225438
    Purpose
    Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.
    Depositor
    Diego Orzaez
    Insert
    CBS-FRT-OCS-BeYDVRepRepA-tNos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225438-plasmid-map-sequence-id-447740

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  8. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

    Plasmid
    #225436
    Purpose
    BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.
    Depositor
    Diego Orzaez
    Insert
    BeYDV geminino 1.0
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225436-plasmid-map-sequence-id-447735

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  9. pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

    Plasmid
    #225430
    Purpose
    OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter
    Depositor
    Diego Orzaez
    Insert
    miniDFR-FRT-OCSterm-FRT-miniDFR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225430-plasmid-map-sequence-id-447730

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  11. pSN993

    Plasmid
    #219961
    Purpose
    Expresses CMT2A mutant version of human KIF1Bß in C. elegans
    Depositor
    Shinsuke Niwa
    Insert
    unc-104 promoter::human KIF1Bß(Q98L) (KIF1B Human, Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219961-plasmid-map-sequence-id-440845

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  13. pLPC-C-SF-TAP-DEST-TLK1- M566T

    Plasmid
    #218831
    Purpose
    Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.
    Depositor
    Travis Stracker
    Insert
    TLK1 M566T (TLK1 Human)
    Use
    Tags
    FLAG-STREP
    Expression
    Mammalian
    Mutation
    M566T
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218831-plasmid-map-sequence-id-436656

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  14. pAG426-GAL-ATXN1-Q85

    Plasmid
    #181712
    Purpose
    Galactose inducible expression of ATXN1-Q85
    Depositor
    Alejandro Chavez
    Insert
    ATXN1-Q85 (ATXN1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    extended repeat of 85 Gln residues replacing nati…
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    181712-plasmid-map-sequence-id-439435

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  15. pUN1309 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pD4H

    Plasmid
    #203901
    Purpose
    Reporter plasmid for the D4H promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Insert
    pD4H-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    203901-plasmid-map-sequence-id-404841

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  16. pSM046

    Plasmid
    #217400
    Purpose
    KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc
    Depositor
    Brian Pfleger
    Article
    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:
    Insert
    BTE2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PTrc
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    217400-plasmid-map-sequence-id-434131

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  17. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid
    #208625
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 72~197, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208625-plasmid-map-sequence-id-433638

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  18. PE-SUMO CRYBA4

    Plasmid
    #217671
    Purpose
    For expression of SUMO tagged human beta A4 crystallin
    Depositor
    Larry David
    Insert
    CRYBA4 (CRYBA4 Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217671-plasmid-map-sequence-id-433253

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  19. pAG416-GAL-FUS-R521C

    Plasmid
    #181718
    Purpose
    Galactose inducible expression of FUS-R521C
    Depositor
    Alejandro Chavez
    Insert
    FUS (FUS Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R521C
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181718-plasmid-map-sequence-id-430958

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  20. pAG416-GAL-FUS-P431L

    Plasmid
    #181717
    Purpose
    Galactose inducible expression of FUS-P431L
    Depositor
    Alejandro Chavez
    Insert
    FUS (FUS Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    P431L
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181717-plasmid-map-sequence-id-430957

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  27. BRAF - R726C in pENTR

    Plasmid
    #216616
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    R726C
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216616-plasmid-map-sequence-id-427247

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  28. BRAF - R671Q in pENTR

    Plasmid
    #216615
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    R671Q
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216615-plasmid-map-sequence-id-427246

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  29. BRAF - R558G in pENTR

    Plasmid
    #216614
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    R558G
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216614-plasmid-map-sequence-id-427243

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  30. BRAF - W531C in pENTR

    Plasmid
    #216613
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    W531C
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216613-plasmid-map-sequence-id-427242

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  31. BRAF - G518C in pENTR

    Plasmid
    #216612
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    G518C
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216612-plasmid-map-sequence-id-427188

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  32. BRAF - S467A in pENTR

    Plasmid
    #216611
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    S467A
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216611-plasmid-map-sequence-id-427172

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  33. BRAF - P453A in pENTR

    Plasmid
    #216610
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    P453A
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216610-plasmid-map-sequence-id-427171

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  34. BRAF - C251Y in pENTR

    Plasmid
    #216609
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    C251Y
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216609-plasmid-map-sequence-id-427170

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  35. BRAF - I208V in pENTR

    Plasmid
    #216608
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    I208V
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216608-plasmid-map-sequence-id-427169

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  36. BRAF - R146W in pENTR

    Plasmid
    #216607
    Purpose
    gateway cloning donor vector containing BRAF ASD mutation
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    R146W
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216607-plasmid-map-sequence-id-427166

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  37. pcDNA3.1-ARL16-His6

    Plasmid
    #216484
    Purpose
    Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor like 16 (ARL16 Human)
    Use
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216484-plasmid-map-sequence-id-426903

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  38. MECP2 in pENTR

    Plasmid
    #216592
    Purpose
    gateway cloning donor vector containing MECP2
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    MECP2 (MECP2 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216592-plasmid-map-sequence-id-426894

    Enlarge

Showing: 6521 - 6560 of 8371 results