We narrowed to 14,168 results for: eng

Showing: 3261 - 3280 of 14168 results

Results per page:

Sort results by:

pYPQ132-STU-Lb

Plasmid

#138099

Purpose

Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
plentiCMV10x-sct-pp65-b2m-HLA-A0201

Plasmid

#196513

Purpose

Express single-chain pMHC with sequencing tag for 10x genomics

Depositor

Howard Chang

Publication

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C, 10xgenomics compatible tag

Promoter

CMV

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF019 MBP-β-catenin-His

Plasmid

#154070

Purpose

Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CTNNB1 (CTNNB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Publication

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_49

Plasmid

#109247

Purpose

pSC101 var7 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.214_mCherry-PP7x12

Plasmid

#205556

Purpose

Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIRECT_10E

Plasmid

#91209

Purpose

Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBDEST.IP

Plasmid

#79472

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

Plasmid

#207862

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGT-Ah5

Plasmid

#122572

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGNW6

Plasmid

#122093

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGNW4

Plasmid

#122088

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGT-Ah9

Plasmid

#122576

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Publication

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Expression

Mammalian

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pACBB-eGFP

Plasmid

#32551

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pCDB179-TurboPETase

Plasmid

#218693

Purpose

Encodes codon optimized TurboPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

TurboPETase

Tags

10xHis

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pP2_tail_fiber

Plasmid

#196332

Purpose

Plasmid expressing P2 tail fiber H and chaperone protein G

Depositor

Baojun Wang

Publication

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

P2 tail fiber H and chaperone protein G

Expression

Bacterial

Promoter

P2 late promoter

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJZC603

Plasmid

#62317

Purpose

sgRNA with 2x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
4784 TRV2-eTnpBc-reRNA4PDS

Plasmid

#251816

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Publication

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBc with guide targeting NbPDS with HDV at 3' end

Tags

AtFDnls

Expression

Plant

Mutation

N4Y/R110K/V192L/L222I

Available since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

Plasmid

#159086

Purpose

Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

Depositor

Jeremy Day

Publication

Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

Insert

empty crRNA-acRNA backbone, dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Promoter

hSYN, U6

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge