We narrowed to 13,996 results for: eng
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Plasmid#91879PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2DepositorAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only
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pMX-HA-UbvG08-IRES-GFP
Plasmid#75030PurposeRetroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)DepositorInsertUbiquitin
UseRetroviralTagsHA and IRES-eGFPMutationUbvG08 with I44A mutation and no terminal GlycinesAvailable SinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAT009
Plasmid#171635PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-B2M
mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterCAG and U6Available SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-mCherry
Plasmid#79509PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDSDepositorInsertmCherry RFP
UseGateway CloningPromoterno PromoterAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGF005
Plasmid#48846PurposeProvides a hygromycin resistance cassette as GreenGate module.DepositorInsertpUBQ10::HygromycinR::tOCS
UseGolden gate compatible cloning vectorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH728-CEN-RLuc/maxCFLuc
Plasmid#38212DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKM492-Myc-His
Plasmid#109283PurposeORBIT integrating plasmid for C-terminal tagging with Myc-His tagDepositorInsertattB-Myc-His
UseOrbit integrating plasmidAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-Fc(IgG1)
Plasmid#145165PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)
Plasmid#114069PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
TagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialPromoterT7lacAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2103b
Plasmid#91062PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRPromoterCmYLCVAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pBAD-eGFP
Plasmid#32553DepositorAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
Tags3xFLAG-tag and His14-tagExpressionBacterialAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PH(Btk)
Plasmid#86635PurposeExpresses eGFP-taggd PH domain of BtkDepositorInsertBtk PH domain (BTK Human)
ExpressionMammalianAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-PDGFRA-reporter
Plasmid#136456PurposeMammalian fluorescent reporter plasmid for PDGFRA signaling.DepositorInsertsPDGFRalpha (PDGFRA Human)
Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMV and Minimal promoter with artificial SRE enha…Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)
Plasmid#114084PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.4DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
ExpressionBacterialPromoterSmR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
HERV-Kcon-RT for Split-PE (pJUL3120)
Plasmid#190107PurposeExpresses HERV-Kcon RT (human codon optimization)DepositorInsertHERV-Kcon-RT
TagsbpNLSExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-HaloTag7
Plasmid#175533PurposeExpression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cellsDepositorInsertLyn11-HaloTag7
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PH1(WT)
Plasmid#86599PurposeExpresses eGFP-tagged PH(WT) domain of human Akt1DepositorInsertAkt1 PH domain (AKT1 Human)
ExpressionMammalianAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only