We narrowed to 39,996 results for: kan

1. hGATA-1 K137R

   Plasmid

   #118353

   Purpose

   This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K137R

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcmv3-STING-C88S-FLAG

   Plasmid

   #198187

   Purpose

   Mammalian expression of FLAG-tagged mouse STING1 C88S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   TMEM173 (Sting1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. hHSF1 K298R

   Plasmid

   #118362

   Purpose

   This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K298R

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRL838

   Plasmid

   #70680

   Purpose

   This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

   Depositor

   Peter Wolk

   Article

   Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

   Type

   Empty backbone

   Use

   Cre/Lox

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330 NL-FKBP-Cas9

   Plasmid

   #162723

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330 N-FKBP-Cas9

   Plasmid

   #162727

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

   Plasmid

   #39228

   Depositor

   Aziz Sancar

   Article

   Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

   Insert

   HERC2 HECT Domain (HERC2 Human)
   

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   C4762A

   Promoter

   T7

   Available Since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pDONR-U6gRNA-PGKpuro2ABFP

   Plasmid

   #136405

   Purpose

   Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Mutation

   BbsI restriction site at position 437 was removed…

   Promoter

   U6, PDK

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MO70-hJunctate

   Plasmid

   #79596

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

   Insert

   junctate (ASPH Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pX330 NC-FKBP-Cas9

    Plasmid

    #162725

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR E2 HSPter-RPS5Ap-mito

    Plasmid

    #163146

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

    Depositor

    Shin-ichi Arimura

    Article

    Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

    Insert

    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
    

    Use

    Entry vector

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MO70 - Orai1 EC-Flag

    Plasmid

    #79590

    Purpose

    Transient expression and retroviral Expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG (inserted between TM3 and TM4 of the protein…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCNL-C1_fibrillarin

    Plasmid

    #65709

    Purpose

    Expresses CNL-fibrillarin in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    fibrillarin (FBL Human)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pX330 L-FKBP-Cas9

    Plasmid

    #162728

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mKikGR-Tubulin-6

    Plasmid

    #57330

    Purpose

    Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mKikGR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pONL-C1_Rab11a

    Plasmid

    #65707

    Purpose

    Expresses ONL-Rab11a in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Rab11a (Rab11a Mouse)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AB.pCCL.sin.cPPT.U6.miR-10a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46581

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-10a Decoy (MIR10A, Mir10a Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

18. hMEF2A K403R

    Plasmid

    #118355

    Purpose

    This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K403R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AB.pCCL.sin.cPPT.U6.miR-375-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46617

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-375 Decoy (MIR375, Mir375 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. MO70-hJunctate - EF-mutant

    Plasmid

    #79597

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    77DAD79 to 77AAA79

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only