We narrowed to 21,797 results for: Sis

1. pCDB179-TfCut2-S121P-D174S-D204P

   Plasmid

   #218695

   Purpose

   Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TfCut2
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   S121P-D174S-D204P

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19_CRISPR_DpmrA

   Plasmid

   #160903

   Purpose

   CRISPR_Cas9, lambda red recombineering

   Depositor

   Anne-Catrin Uhlemann

   Article

   McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

   Inserts

   Cas9
   Lambda Red Recombineering Genes
   Zeocin Resistance Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. peCALWY-4

   Plasmid

   #22236

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-4 Zn(II) sensor (ATOX1 Human, GFP's)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C418S mutation linker consists of 9 GGSGGS repea…

   Promoter

   Available since

   Nov. 25, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSH124-ssr

   Plasmid

   #198025

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Ap/Cb resistance cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ApR promoter

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSH424-ssr

   Plasmid

   #198026

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Sm resistance cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SmR promoter

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-FLEX-SaCas9-U6-sgSlc18a2

   Plasmid

   #124849

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

   Plasmid

   #124862

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gRNA NIA Null

   Plasmid

   #198728

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CIB81-pmGFP

    Plasmid

    #75366

    Purpose

    Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    prenylated EGFP

    Expression

    Mammalian

    Mutation

    residues 1-81 of CIB1

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hHSF1 K80R

    Plasmid

    #118350

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to R.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWJB9

    Plasmid

    #126650

    Purpose

    Expresses AdrA from a modified pLAFR3

    Depositor

    Ute Romling

    Article

    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

    Insert

    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCB24 pDEST crybb1:TagBFP

    Plasmid

    #195987

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Markus Affolter

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3xFLAG-dCas9/pMSCVhyg

    Plasmid

    #134323

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGE334

    Plasmid

    #153243

    Purpose

    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGE337

    Plasmid

    #153246

    Purpose

    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO GFP DNAJB1

    Plasmid

    #19495

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. plRL58

    Plasmid

    #166886

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge