We narrowed to 14,580 results for: pET

1. pETSyt1C2AB(80 JuxtaK-421)msGFP

   Plasmid

   #209011

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-HypaSpCas9-NLS-6xHis

   Plasmid

   #207384

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-evoSpCas9-NLS-6xHis

   Plasmid

   #207389

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28 sf:em-blue-shift.1

   Plasmid

   #191928

   Purpose

   Encodes a GFP design, sf:em-blue-shift.1

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:em-blue-shift.1
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S Q69P S72A T108V Y145M T167V V224I

   Promoter

   T7

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28b(+)/ADK C77S

   Plasmid

   #201409

   Purpose

   Overexpresses Escherichia coli adenylate kinase C77S

   Depositor

   Gary Pielak

   Article

   Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

   Insert

   Escherichia coli adenylate kinase C77S (adk Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed cysteine 77 to serine

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET24-aCatenin(82-906)-His6

   Plasmid

   #203332

   Purpose

   Bacterial expression of wild-type alpha-catenin

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 82-906

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-51b(+) ChemoB-CaM

    Plasmid

    #193812

    Purpose

    Protein production of ChemoB-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-CaM
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EBFP2: N39Y, V206K

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-51b(+) ChemoC-CaM

    Plasmid

    #193813

    Purpose

    Protein production of ChemoC-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoC-CaM
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-51b(+) ChemoY-CaM

    Plasmid

    #193814

    Purpose

    Protein production of ChemoY-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoY-CaM
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Venus:A206K

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-51b(+) ChemoR-CaM

    Plasmid

    #193815

    Purpose

    Protein production of ChemoR-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoR-CaM
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    mScarlet: D201K

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-TRE-YPet-WPRE

    Plasmid

    #193335

    Purpose

    Expresses YPet under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a LZ LED 4CA-FLAG

    Plasmid

    #199681

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED 4CA (GPR155 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    C595.604.629.638 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a LZ LED Y551A-FLAG

    Plasmid

    #199680

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED Y551A (GPR155 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Y551 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM-41P-DmGW182_744-817_N

    Plasmid

    #147064

    Purpose

    Bacterial Expression of DmGW182_744-817

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182_744-817 (gw Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one non silent mutations N799S and one silent mut…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM-60-NcQDE2_506-938-D603K_M

    Plasmid

    #146962

    Purpose

    Bacterial Expression of NcQde-2_506-938-D603K

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-D603K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM-60-NcQDE2_506-938-K638E_M

    Plasmid

    #146963

    Purpose

    Bacterial Expression of NcQde-2_506-938-K638E

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    NcQde-2_506-938-K638E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM-41P-HsNot2_344-540_M

    Plasmid

    #146946

    Purpose

    Bacterial Expression of HsNot2_344-540

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

    Insert

    HsNot2_344-540 (CNOT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge