We narrowed to 12,311 results for: BASE

1. qTAG-C-miniTurbo-Blast

   Plasmid

   #207714

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. mEGFP-8xbiRhoBAST

   Plasmid

   #196351

   Purpose

   Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

   Depositor

   Murat Sunbul

   Article

   Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

   Insert

   mEGFP-8xbiRhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TALED_Left-ND1-E1347A

   Plasmid

   #183895

   Purpose

   Expresses TALED_Left-ND1-E1347A in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB8_4-3i-ATP8_v8_AGKS

   Plasmid

   #191513

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE 4-3i-ATP8 AGKS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB5_R8-3i-ATP8_v8_AGKS

   Plasmid

   #191510

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE R8-3i-ATP8 AGKS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-C-miniTurbo-Puro

   Plasmid

   #207725

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. qTAG-C-V5-Puro

   Plasmid

   #207730

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCas3

   Plasmid

   #229538

   Purpose

   pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Insert

   Cas3
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Puro-miRFP670nano3

   Plasmid

   #207691

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. 4xMS2-hTR

    Plasmid

    #154940

    Purpose

    4xMS2 stem-loop-hTR-500 bp genomic sequence

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    4xMS2 stem-loop-hTR-500 bp genomic sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid

    #189871

    Purpose

    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK1 (NEK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-C-mNeon-Blast

    Plasmid

    #207709

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCRi

    Plasmid

    #207524

    Purpose

    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    

    Use

    Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-C-ultraID-Zeo

    Plasmid

    #207737

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET-APOBEC1-YTH

    Plasmid

    #200158

    Purpose

    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTH
    

    Use

    Tags

    6xHis, HA, and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-C-moxGFP-Zeo

    Plasmid

    #207732

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. ZipGFP1-10_TEV

    Plasmid

    #81242

    Purpose

    Zipped GFP1-10 with TEV cleavage sequence T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    zipped GFP11 with TEV cleavage sequence T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-NP-B/BRISBANE/60/2008

    Plasmid

    #187921

    Purpose

    Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    UNTAGGED

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSplit2-NO-PRPF6

    Plasmid

    #51740

    Purpose

    Express LucN-PRPF6 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    PRP6 (PRPF6 Human)
    

    Use

    Tags

    FLAG and Luciferase (1-398aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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