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pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

Plasmid

#221819

Purpose

Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

GFP11-HA donor with homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

Plasmid

#221835

Purpose

Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

2xFLAG with dSERT homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

Plasmid

#221832

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1AR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221833

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221837

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

Plasmid

#221838

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

Plasmid

#221839

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-Fc-pST3Gal1 Dead

Plasmid

#228821

Purpose

Expresses Fc-fusion pST3Gal1 Dead (Q108A/Y233A/Y269F) in mammalian cells

Depositor

Sriram Neelamegham

Article

Hombu et al Nat Commun. 2025 Jul 18;16(1):6637. doi:

Insert

Fc-pST3Gal1 Dead

Use

Lentiviral

Tags

6His tag and human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted and Q10…

Promoter

CMV

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVTHM-Chim-Pchi-3xFLAG

Plasmid

#231536

Purpose

Expresses Salpingoeca helianthica chimeric Pchi in mammalian cells, for protein overexpression

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG flanked with N- and C-termini of mSox2

Tags

3xFLAG

Expression

Mammalian

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLVTHM-Chim-Salhel-I-3xFLAG

Plasmid

#231535

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I in mammalian cells, for protein overexpression

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG flanked with N- and C-termini of mSox2

Tags

3xFLAG

Expression

Mammalian

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pUB_SM-FLuc0_EMCV-Ren

Plasmid

#210590

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Inserts

Spaghetti monster (FLAG)-nonoptimal FLuc
Optimal Renilla luciferase

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

EMCV IRES and UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-FLuc100_EMCV-Ren

Plasmid

#210591

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Inserts

Spaghetti monster (FLAG)-optimal FLuc
Optimal Renilla luciferase

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

EMCV IRES and UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13A

Plasmid

#224408

Purpose

Mammalian expression of Kif 13a

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13a (Kif13a Mouse)

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.Mark2-3myc

Plasmid

#224416

Purpose

Mammalian expression of MARK2

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

MARK2 (MARK2 Human)

Tags

3myc

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-GAC site)

Plasmid

#170149

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(GAC)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (RAB7A-GAC site)

Plasmid

#170140

Purpose

AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-RAB7A(GAC)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-PTK6-YF

Plasmid

#210715

Purpose

Construct for stable expression of constitutively active human PTK6 in mammalian cells.

Depositor

Angela Tyner

Article

Zheng et al Mol Cell Biol. 2010 Sep;30(17):4280-92.

Insert

Protein tyrosine kinase 6 (PTK6 Human)

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMX_mePOU

Plasmid

#206396

Purpose

Expresses mouse ePOU in mammalian cells, for retrovirus generation.

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+W275L

Plasmid

#196021

Purpose

Expresses CRKL W160L + W275L

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pVAX1-VSVG-INDIANA2

Plasmid

#196623

Purpose

Express VSV-G protein in mammalian cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

VSV-G

Expression

Mammalian

Mutation

WT

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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