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pFA6a-kanMX6-P81nmt1-mYFP

Plasmid

#105144

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mYFP

Tags

81nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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GST-auxilin(547-910)

Plasmid

#62572

Purpose

Codes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruption

Depositor

Ernst Ungewickell

Article

Holstein et al J Cell Biol. 1996 Nov;135(4):925-37.

Insert

Auxilin

Tags

GST

Expression

Bacterial

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only
HOXD10-pSG5

Plasmid

#21008

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

HOXD10 (HOXD10 Human)

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pDOC-K-glmS-lacIZ

Plasmid

#158061

Purpose

Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

lacIZ operon

Use

Synthetic Biology

Expression

Bacterial

Promoter

lac

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-N-22

Plasmid

#57420

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pI4-MTU-DO-URA

Plasmid

#160025

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pICH75111

Plasmid

#50327

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, beta-glucuronidase (E. coli) with two introns,

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSHS293

Plasmid

#87348

Purpose

D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/D435C/C574S/E945C

Promoter

T7

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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tdEos-Talin-18

Plasmid

#57672

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pICH42211

Plasmid

#50255

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-zeoCV

Plasmid

#73890

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

zeoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2

Plasmid

#118853

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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tdEos-Paxillin-22

Plasmid

#57653

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRRL.gp91.EF1aIntron.eGFP

Plasmid

#30466

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/EF1aIntron/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Tags

1D4

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJ291_WT_med

Plasmid

#128856

Purpose

Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFTK088

Plasmid

#171360

Purpose

AMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

AMA1 sequence (short), entry vector providing fungal replication

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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