We narrowed to 14,031 results for: TIM

1. CRY2olig-mCherry

   Plasmid

   #60032

   Purpose

   Expresses a fusion of CRY2PHR E490G with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2PHR E490G (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet28a-His6-Keap1

   Plasmid

   #62454

   Purpose

   His6 tagged Keap1 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   His-Tag

   Expression

   Bacterial

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-MS2coatProtein

   Plasmid

   #103832

   Purpose

   Live cell reader for MS2 loop RNA production

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   mCherry-MS2 coat protein (cp )
   

   Expression

   Mammalian

   Mutation

   MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICH41331::pU6cm-pegR::EURb7

   Plasmid

   #227695

   Purpose

   Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   Altered epegRNA backbone with cloning sites for gRNA and RTT.
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   U6 composite

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMZ827

   Plasmid

   #155321

   Purpose

   Constitutive expression of E-PIF6(1-100) in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-E-PIF6(1-100)-NLS-Tnos
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROF051

   Plasmid

   #155328

   Purpose

   Constitutive expression of SRDX-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-SRDX-NLS-EL222-Tnos
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pROF021

   Plasmid

   #155326

   Purpose

   Expression of PULSE-controlled FLuc

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
   

   Use

   Luciferase and Synthetic Biology; Eukaryotic expr…

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-HiBiT-CD81

    Plasmid

    #162593

    Purpose

    N-terminally HiBiT-tagged human CD81 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD81 (CD81 human, Human, Synthetic)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYSD061

    Plasmid

    #212915

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

    Plasmid

    #86424

    Purpose

    PCR tagging module with yeast codon optimized mTurquoise2

    Depositor

    Sue Jaspersen

    Article

    Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

    Insert

    mTurquoise2
    

    Expression

    Yeast

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. KA1396_pGL4-UBCmin-luc2/Klf4-E'

    Plasmid

    #124187

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KA2626_pGL4-UBCmin-luc2/Klf2-D'

    Plasmid

    #124199

    Purpose

    Luciferase reporter driven by the Klf2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf2 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. KA1401_pGL4-UBCmin-luc2/Klf4-F'

    Plasmid

    #124188

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KA1395_pGL4-UBCmin-luc2/Klf4-B'

    Plasmid

    #124186

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYSD100

    Plasmid

    #212924

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLS-SceI

    Plasmid

    #137725

    Purpose

    Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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