4,142 results

1. pZW6 (CK2alpha)

   Plasmid

   #27086

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPAGFP-C1

   Plasmid

   #11910

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   PAGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQCXIH-Myc-YAP-5SA

   Plasmid

   #33093

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S61A, S109A, S127A, S164A, S381A (phosphorylation…

   Promoter

   CMV/MSV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCI-neo beta catenin WT

   Plasmid

   #16518

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   beta catenin (CTNNB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T7-RelA

   Plasmid

   #21984

   Depositor

   Warner Greene

   Article

   Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

   Insert

   NFkB p65 subunit (RELA Human)
   

   Use

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   For more details for this expression construct,…

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pCI-SEP_NR2B

   Plasmid

   #23998

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   NR2B (Grin2b Rat)
   

   Use

   Tags

   NR2B signal sequence (aa1-31) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.3_LIN28A

   Plasmid

   #26819

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-LIN28A-3'UTR (LIN28A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pBABE BRG1

   Plasmid

   #1959

   Depositor

   Robert Kingston

   Article

   Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

   Insert

   BRG1 (SMARCA4 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   E1262K R1575Q

   Promoter

   Available since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. cytoplasmic EKAR (EGFP-mRFP)

   Plasmid

   #18680

   Depositor

   Karel Svoboda

   Article

   Harvey et al PNAS 2008 Dec 9;105(49):19264-9

   Insert

   ERK Activity Reporter
   

   Use

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-HA-JMJD2B

    Plasmid

    #24181

    Purpose

    Mammalian expression of human JMJD2B with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    JMJD2B (KDM4B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cdk2-HA

    Plasmid

    #1884

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk2 (CDK2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 12XCSL-DsRedExpressDL

    Plasmid

    #47683

    Purpose

    Notch reporter

    Depositor

    Urban Lendahl

    Article

    Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

    Insert

    TP-1 promoter
    

    Use

    Notch reporter construct

    Tags

    dsRedExpressDR

    Expression

    Mutation

    hexamerized 50-bp EBNA2 response element of the T…

    Promoter

    NA

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p-cyclinB1-mcherry (1958)

    Plasmid

    #26063

    Depositor

    Jonathon Pines

    Article

    Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Melissa Harrison, Jill Wildonger, Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6M-Pak1 K299R

    Plasmid

    #12210

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive Pak1: K299R

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMLS-SV40-EGFP

    Plasmid

    #46919

    Purpose

    Target EGFP gene that is stably integrated into HEK293 genome

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    GFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLOVE-Nmyc

    Plasmid

    #15951

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

    Insert

    v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

19. nls-NgAgo-GK

    Plasmid

    #78253

    Purpose

    Mammalian expression of NgAgo (Argonaute from N. gregoryi).

    Depositor

    Chunyu Han

    Article

    Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    NgAgo
    

    Use

    Tags

    a nuclear localization signal (NLS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

    Plasmid

    #141379

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge