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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,239 results for: FIE

Showing: 6601 - 6640 of 8239 results

  4. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid
    #133318
    Purpose
    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pasteurianus)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    133318-plasmid-map-sequence-id-266833

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  5. pPEE17

    Plasmid
    #128361
    Purpose
    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Insert
    Acetamidase (amdS)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128361-plasmid-map-sequence-id-267942

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  12. pUAS-hActin-luc2

    Plasmid
    #48081
    Purpose
    Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    removed hsp70 promoter, inserted amplified 615 bp…
    Promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. Nup54 no FG-mEGFP494(0)

    Plasmid
    #164269
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP
    Depositor
    Sanford Simon
    Article
    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164269-plasmid-map-sequence-id-325395

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  19. pFBCPDL6

    Plasmid
    #67285
    Purpose
    CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression
    Depositor
    Aziz Sancar
    Article
    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.
    Insert
    CPD photolyase (phr Fly)
    Use
    Bacterial vector, insert not expressed
    Tags
    Flag
    Expression
    Mutation
    V275E*
    Promoter
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  20. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  28. pFTK093

    Plasmid
    #171365
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171365-plasmid-map-sequence-id-352204

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  29. pENTR-THT III

    Plasmid
    #55791
    Purpose
    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Type
    Empty backbone
    Use
    RNAi; Gateway
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    modified H1 RNA gene
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55791-plasmid-map-sequence-id-89573

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  31. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid
    #168207
    Purpose
    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection
    Depositor
    Markus Hafner
    Article
    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.
    Type
    Empty backbone
    Use
    Unspecified; Pdest gateway
    Tags
    V5 APEX2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168207-plasmid-map-sequence-id-331104

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  34. pET:GACG:GFP

    Plasmid
    #60735
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60735-plasmid-map-sequence-id-97721

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Showing: 6601 - 6640 of 8239 results