We narrowed to 4,380 results for: 1-Oct;

1. pAND_Int2

   Plasmid

   #60590

   Purpose

   A transcriptional AND circuit constructed by layering NOR and NOT gates

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   AND_Int2
   

   Expression

   Bacterial

   Promoter

   PPhlF

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAND_yfp

   Plasmid

   #60592

   Purpose

   used with pSpec to generate an AND gate without memory

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   AND_yfp
   

   Expression

   Bacterial

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpec

   Plasmid

   #60593

   Purpose

   used with pAND_YFP to generate an AND gate without memory

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Spec
   

   Expression

   Bacterial

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCis_7+8

   Plasmid

   #60587

   Purpose

   a 2-integrase plasmid

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cis_7+8
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCis_2+5

   Plasmid

   #60586

   Purpose

   a 2-integrase plasmid

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cis_2+5
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-a(+)-6xHis-miRFP(-5)-HO1

   Plasmid

   #219424

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Inserts

   miRFP682
   HO1
   

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   Contains Ribosomal Binding Site for expression of…

   Promoter

   T7

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-hMLH1dn for IVT

   Plasmid

   #178114

   Purpose

   Template for in vitro transcription of human MLH1dn

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized) (MLH1 Human)
   

   Use

   Template for in vitro transcription

   Mutation

   Detailed in manuscript

   Promoter

   T7 (inactivated)

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29b-rhMOG

   Plasmid

   #225484

   Purpose

   Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

   Depositor

   John Pak

   Article

   Wu et al J Immunol Methods. 2024 Oct 13:113764. d

   Insert

   Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-29 (signal peptide) and 150…

   Promoter

   T7

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCP-NMS

    Plasmid

    #210705

    Purpose

    This plasmid express EF1a promoter driven MCP-NMS transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MCP-eN3x9

    Plasmid

    #210706

    Purpose

    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MCP-3x9aaTAD

    Plasmid

    #210707

    Purpose

    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P POLA1_2

    Plasmid

    #160790

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P POLA1_1

    Plasmid

    #160789

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPing

    Plasmid

    #47100

    Purpose

    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

    Depositor

    Wayne Parrott

    Article

    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

    Inserts

    Ping cDNA
    mPing
    hygromycin resistance gene
    

    Use

    Plant expression

    Tags

    GFP

    Expression

    Yeast

    Promoter

    CaMV35s and StUbi3

    Available Since

    Sept. 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. AAV-hSyn-SpdCas9-W3SL

    Plasmid

    #210708

    Purpose

    This Plasmid express hSyn promoter driven SpdCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    S. Pyogenes dCas9
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    hSyn

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1-human LIC1 C-terminal half

    Plasmid

    #74599

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    T7

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-MS2V5-PABPC1

    Plasmid

    #65807

    Purpose

    expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PAB1 (PABPC1 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET41b+_Npl4

    Plasmid

    #117108

    Purpose

    Production of recombinant Npl4 in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Npl4 (NPLOC4 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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