We narrowed to 22,070 results for: ESC

1. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

   Plasmid

   #217768

   Purpose

   Fluorescent reporter for ceramide (for stable mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Sleeping beauty transposase-compatible genome ins…

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CA3 domain aa 317-400 plus vector-based linker LE…

   Promoter

   EF-1alpha promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

   Plasmid

   #217767

   Purpose

   Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400

   Promoter

   T7 promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

   Plasmid

   #217766

   Purpose

   Fluorescent reporter for ceramide (for bacterial expression and purification)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400 plus vector-based linker LE…

   Promoter

   T7 promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

   Plasmid

   #172618

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr283Ala

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-834_NY-ESO-1_TCR_FAS/IL4R

   Plasmid

   #207497

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/IL4R, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-FLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172619

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CaMKII-Archon1-KGC-EGFP-ER2

   Plasmid

   #108419

   Purpose

   AAV production plasmid encoding for Archon1 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. px458-CCND1

   Plasmid

   #172630

   Purpose

   Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against CCND1 (CCND1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. shERK2b-mlpx-puro

   Plasmid

   #65230

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

    Plasmid

    #172647

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-FLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172626

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherry

    Plasmid

    #172639

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. shERK2a-mlpx-puro

    Plasmid

    #65229

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-Archon2-KGC-EGFP-ER2

    Plasmid

    #108421

    Purpose

    AAV production plasmid encoding for Archon2 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIG-836_NY-ESO-1_TCR_FAS/OX40

    Plasmid

    #207499

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/OX40, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

    Plasmid

    #172609

    Purpose

    Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)
    

    Use

    CRISPR; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIG-833_NY-ESO-1_TCR_FAS/CD28

    Plasmid

    #207496

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/CD28, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol2-UAS-zArchon2-KGC-EGFP-ER2

    Plasmid

    #108428

    Purpose

    Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon2-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. shERK1a-mlpx-puro

    Plasmid

    #65228

    Purpose

    encodes a shRNA against ERK1

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid

    #85844

    Purpose

    Genetically encoded voltage sensor ArcLight- codon optimized

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    ArcLightCo-Q239
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    hSyn1

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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