We narrowed to 6,087 results for: nls

1. ipUSEPR-sg-Hs-PHGDH-2

   Plasmid

   #188676

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SGEP-sh-Hs-PHGDH-1967

   Plasmid

   #188672

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ipUSEPR-sg-Hs-PHGDH-5

   Plasmid

   #188679

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ipUSEPR-sg-Hs-PHGDH-6

   Plasmid

   #188680

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ipUSEPR-sg-Hs-PHGDH-4

   Plasmid

   #188678

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ipUSEPR-sg-Hs-PHGDH-3

   Plasmid

   #188677

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-ABE8eWQ

   Plasmid

   #161815

   Purpose

   Expresses ABE8eWQ in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL1180-HR-PUbECFP

   Plasmid

   #47917

   Purpose

   Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

   Depositor

   Leslie Vosshall

   Insert

   ECFP
   

   Use

   Tags

   NLS

   Expression

   Insect

   Mutation

   Promoter

   Aedes aegypti polyubiquitin (PUb)

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX380 Treponema denticola Cas9

   Plasmid

   #68317

   Purpose

   Treponema denticola Cas9

   Depositor

   Feng Zhang

   Insert

   TdCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TC9nickpAB

    Plasmid

    #71311

    Purpose

    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9 nickase
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    D10A nickase

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ipUSEPR-sg-Hs-PHGDH-int2

    Plasmid

    #188682

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid

    #188681

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ipUSEPR-sg-Hs-PHGDH-int3

    Plasmid

    #188683

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-SasgRNA scaffold-CMV-nSaCas9

    Plasmid

    #171695

    Purpose

    Expresses nSaCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    sgRNA scaffold-nSaCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458-3xHA-FnCas9

    Plasmid

    #130969

    Purpose

    3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    FnCas9
    

    Use

    CRISPR

    Tags

    3xHA-NLS and NLS-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid

    #220611

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM2AH + Slo1

    Plasmid

    #16195

    Depositor

    Larry Salkoff

    Article

    McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

    Insert

    calcium-activated potassium channel (KCNMA1, Kcnma1 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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