We narrowed to 164,247 results for: HTT

1. pJK556

   Plasmid

   #74277

   Purpose

   Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-1246 to serine, numerous silent m…

   Promoter

   T7

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

   Plasmid

   #191389

   Purpose

   Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hCdt1(1-100)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hCas9D10A

   Plasmid

   #52101

   Purpose

   The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter

   Depositor

   Izuho Hatada

   Insert

   hCas9D10A
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available Since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

4. mEmerald-MyosinIIB-C-18

   Plasmid

   #54192

   Purpose

   Localization: Cytoskeleton, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   MyosinIIB (MYH10 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET His6 MBP N10 TEV LIC cloning vector (2C-T)

   Plasmid

   #29706

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 NT3

   Plasmid

   #39981

   Depositor

   Yves-Alain Barde

   Insert

   Neurotrophin 3 (Ntf3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-SUN1 mScarlet

   Plasmid

   #187683

   Purpose

   In vivo visualization of SUN1

   Depositor

   Mary Beckerle

   Insert

   SUN1 (Sun1 Mouse)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-RNAse H

   Plasmid

   #165575

   Purpose

   Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   RNAse H
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. R777-E229 Hs.RASA2

   Plasmid

   #70513

   Purpose

   Gateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RASA2 (RASA2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET Trx His6 LIC cloning vector (2Tc-T)

    Plasmid

    #37239

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thioredoxin (Trx)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. R777-E235 Hs.RASAL2

    Plasmid

    #70519

    Purpose

    Gateway ORF clone of human RASAL2 [NM_170692.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASAL2 (RASAL2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22B_SA_PcrA

    Plasmid

    #102999

    Purpose

    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker

    Depositor

    Martin Webb

    Insert

    SA_PcrA
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    PcrA 199-2423

    Promoter

    T7

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOpen-T5gene122

    Plasmid

    #165544

    Purpose

    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    T5 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. mEos2-PSD95-N-14

    Plasmid

    #57411

    Purpose

    Localization: Membrane Scaffold, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    PSD95
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. FUGW-H2B-mCherry

    Plasmid

    #132333

    Purpose

    Third generation lentiviral vector for hUbC-driven H2B-mCherry expression

    Depositor

    Bjoern Schwer

    Insert

    H2B-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    July 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDLxR6

    Plasmid

    #172560

    Purpose

    Inducible gene expression, pCDF origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LT3GEPIR-NEK7shRNA1

    Plasmid

    #220550

    Purpose

    To inducibly knockdown NEK7 expression

    Depositor

    Sean Lee

    Insert

    NEK7 shRNA1 (NEK7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-TET-PH-lox66FRT

    Plasmid

    #139561

    Purpose

    piggyBac expression vector with TET-ON system and puromycin selection

    Depositor

    Minoru Ko

    Insert

    PGK-puroR-pA cassette, inserted in PB-TET vector
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBScomp High

    Plasmid

    #223202

    Purpose

    Group B Streptococcus High Expression Modular Complementation Vector

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    Complementation vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBScomp Low

    Plasmid

    #223203

    Purpose

    Group B Streptococcus Low Expression Modular Complementation Vector

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    Complementation vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-TET-ZH-lox66FRT

    Plasmid

    #139563

    Purpose

    piggyBac expression vector with TET-ON system and Zeocin selection

    Depositor

    Minoru Ko

    Insert

    PGK-ZeoR-pA cassette, inserted in PB-TET vector
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PB-TET-BH-lox66FRT

    Plasmid

    #139562

    Purpose

    piggyBac expression vector with TET-ON system and Blasticidin selection

    Depositor

    Minoru Ko

    Insert

    PGK-BSD-pA cassette, inserted in PB-TET vector
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ECFP-Peroxisomes-0

    Plasmid

    #54548

    Purpose

    Localization: Peroxisomes, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    ECFP and Peroximal Targeting Signal 1 (PTS1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pFBOH-LIC

    Plasmid

    #26099

    Purpose

    SGC Empty baculovirus expression/transfer vector

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    6x His and Thrombin protease cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pColdII-SIRPT1-Eptesicus fuscus

    Plasmid

    #78113

    Purpose

    Nt His-tag Eptesicus fuscus Sacsin 89-1368

    Depositor

    Kalle Gehring

    Insert

    Sacsin Eptesicus fuscus 89-1368
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLL39

    Plasmid

    #15458

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    
    

    Use

    S. aureus-e. coli shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Nesprin3-C-18

    Plasmid

    #58194

    Purpose

    Localization: Nuclear Membrane , Excitation: N/A, Emission: N/A

    Depositor

    Michael Davidson

    Insert

    Nesprin3 (SYNE3 Human)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. PS-CFP2-Lifeact-7

    Plasmid

    #54695

    Purpose

    Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    PS-CFP2-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

29. lentiGuide_puro-P2A-mNeonGreen

    Plasmid

    #221464

    Purpose

    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker

    Depositor

    James Eshleman

    Insert

    mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6, EF-1a

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPB-spCas9-pNeo

    Plasmid

    #214120

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCherry-MannII-N-10

    Plasmid

    #55074

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    MannII
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

32. p(+)MV-NSE-FlagP-F497A-3p

    Plasmid

    #58796

    Purpose

    Expresses recombinant measles virus with Flag-P-F497A gene tagged si2 (siP) target sequence

    Depositor

    Branka Horvat

    Insert

    Measles virus antigenome
    

    Use

    Tags

    Flag

    Expression

    Mutation

    MeV-P F497A

    Promoter

    T7

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Peripherin/pBSSK+

    Plasmid

    #98132

    Purpose

    Peripherin (NM_006262) in pBSSK+

    Depositor

    Rita Shiang

    Insert

    Peripherin (PRPH Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pHAGE2-EF1aL-dsRed-W

    Plasmid

    #195863

    Purpose

    lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

    Depositor

    Darrell Kotton

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed

    Expression

    Mutation

    Promoter

    EF1aL

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRIPZ (M)-HT-NLS

    Plasmid

    #82518

    Purpose

    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    Nuclear localizaition sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV5B-TGFbeta receptor II wt (N-term HA)

    Plasmid

    #24801

    Depositor

    Jeff Wrana

    Insert

    TGFbeta receptor II (TGFBR2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

37. mCherry-Nucleus-7

    Plasmid

    #55110

    Purpose

    Localization: Nucleus, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Use

    Tags

    mCherry-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pCDH-CMV-Nluc

    Plasmid

    #73038

    Purpose

    Express Nluc reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-53-RtcA

    Plasmid

    #107876

    Purpose

    Expresses E. coli RtcA in bacterial expression cells

    Depositor

    Jay Hesselberth

    Insert

    E. coli RtcA (rtcA Escherichia coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV_Zika_Cv_Flag

    Plasmid

    #79628

    Purpose

    Third generation Self-inactivating lentivector expressing Cv from Zika virus

    Depositor

    Vaithi Arumugaswami

    Insert

    Cv
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge