We narrowed to 7,278 results for: 11

1. pPuroR-MCS-LexA-VP16-MiniWhite

   Plasmid

   #165898

   Purpose

   Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable Empty Enhancer Driver
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbB8k-csg-NbMS2

   Plasmid

   #171701

   Purpose

   Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.

   Depositor

   Neel Joshi

   Article

   Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

   Insert

   csgBACEFG, with nanobody fused to CsgA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PML-ER-pBabe

   Plasmid

   #73556

   Purpose

   vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   PML-IV (PML Human)
   

   Use

   Retroviral

   Tags

   ER

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

   Plasmid

   #140202

   Purpose

   CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology; Proof-of-concept te…

   Mutation

   G174D

   Promoter

   PelcA

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEasyG3_nat

   Plasmid

   #184918

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEasyG3_mic

   Plasmid

   #184920

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. VQR variant

   Plasmid

   #138560

   Purpose

   Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PDF1::mCitrine-MBD

   Plasmid

   #115222

   Purpose

   Microtubule visualization

   Depositor

   Jan Traas

   Article

   Armezzani et al Development. 2018 Jun 4;145(11). pii: de

   Insert

   Microtubule binding domain
   

   Tags

   mCitrine

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-REDD1 77-232 (#426)

   Plasmid

   #99921

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 77-232

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALEN-CCR5R18-CtermQ7

    Plasmid

    #51443

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) Q7 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK4a_020

    Plasmid

    #123833

    Purpose

    Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    d4PEST tag
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5 Flag-Smad1 (4SP/AP)

    Plasmid

    #14043

    Depositor

    Joan Massague

    Article

    Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

    Insert

    Smad1 (4SP/AP) (SMAD1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Four Serine to Alanine mutations in the linker re…

    Available Since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

13. MTK5_012

    Plasmid

    #123856

    Purpose

    Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector RE'-A1 Insulator
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB1-PRD(703-800)-Strep

    Plasmid

    #141344

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

    Insert

    GB1-His6-TEV-ALIX(703-800)-Strep
    

    Tags

    GB1-His6-TEV and Strep-tag (WSHPQFEK)

    Expression

    Bacterial

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-HypaCas9-TUBA1B_sgRNA

    Plasmid

    #183889

    Purpose

    pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATF4 10: 5’ATF4.uORF1.GFP

    Plasmid

    #21857

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    upstream ORF1 (uORF) of ATF4 fused to EGFP

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cx43-sfGFP

    Plasmid

    #70053

    Purpose

    Expresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Tags

    non-monomerized superfolder GFP (V207)

    Mutation

    fused in frame to non-monomerized superfolder GFP

    Promoter

    CMV

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK0_030

    Plasmid

    #123969

    Purpose

    Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    phiC31-hygro-mRuby2PEST landing
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDK4mut(K35M)-3XFlag-pBabe

    Plasmid

    #73559

    Purpose

    vector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK4mut(K35M)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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