We narrowed to 8,419 results for: BLI;

1. dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo

   Plasmid

   #137712

   Purpose

   Vector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA telo

   Depositor

   Dominik Niopek

   Insert

   dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS-S-B.1.1.7-FLAG

   Plasmid

   #177093

   Purpose

   B.1.1.7 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.1.7 (S SARS-CoV-2)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmr101A-cPr-mNeonGreen (KmR)

   Plasmid

   #177209

   Purpose

   Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Yeast codon optimized

   Promoter

   Synthetic constitutive promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PX406 Pasteurella multocida Cas9

   Plasmid

   #68703

   Purpose

   Pasteurella multocida Cas9

   Depositor

   Feng Zhang

   Insert

   PmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)

   Plasmid

   #48286

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mT2A-td-sfGFP-FNF

   Plasmid

   #182339

   Purpose

   Recombineering cassette plasmid for T2A-td-sfGFP-FNF

   Depositor

   Mario Capecchi

   Insert

   T2A-td-sfGFP-FNF
   

   Use

   Mouse Targeting; Flp / frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TP73

   Plasmid

   #39077

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TP73 (TP73 Human)
   

   Use

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pJK551

   Plasmid

   #72425

   Purpose

   Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase, N-terminal His6 tag (H6AaUctD)

   Depositor

   T. J. Kappock

   Insert

   putative CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO/Flag-mKO2

    Plasmid

    #110371

    Purpose

    Mammalian expression of mKO2 with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    monomeric Kusabira Orange 2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GGPS1

    Plasmid

    #38855

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GGPS1 (ggps1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. TC9nickpAB

    Plasmid

    #71311

    Purpose

    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9 nickase
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    D10A nickase

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIC65

    Plasmid

    #63061

    Purpose

    Retroviral expression of EGFP-TEV-S-tag Mis12 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    hMis12, IMAGE clone 3351245 (MIS12 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    L68R

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK406

    Plasmid

    #72420

    Purpose

    Produces Acetobacter aceti 1023 Est05, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    hypothetical protein
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…

    Promoter

    T7

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-HLA-B15-His

    Plasmid

    #104837

    Purpose

    HLA-B150101 cloned as a His tag

    Depositor

    Sébastien Walchli

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Entry vector for gateway system

    Tags

    his

    Expression

    Mutation

    2 differences compared to original B15: L2->R …

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS L30 mRuby3-Rab5c

    Plasmid

    #166426

    Purpose

    Rab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab5C
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMIY-Arg1-Flag

    Plasmid

    #34581

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

19. RGS10

    Plasmid

    #39138

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RGS10 (RGS10 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pSG5-Bcr

    Plasmid

    #39855

    Depositor

    Nora Heisterkamp

    Insert

    BCR (BCR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only