We narrowed to 7,122 results for: GFP expression plasmids

1. pHIV-EGFP.Flag-EZH2-mt2

   Plasmid

   #220245

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-EGFP.Flag-EZH2-mt1

   Plasmid

   #220244

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 1 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R27A, R31A, F165A

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn-Chronos-GFP

   Plasmid

   #59170

   Purpose

   AAV-mediated expression of Chronos-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIZ-EGFP-MYO10-HMM

   Plasmid

   #87257

   Purpose

   Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Expression

   Insect

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   OpIE2

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKII-Chronos-GFP

   Plasmid

   #58805

   Purpose

   AAV expression of Chronos-GFP under the CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMT-MjtRNA-GFP(47TAG)

   Plasmid

   #193251

   Purpose

   Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Inserts

   GFP(47TAG)
   MjtRNA
   

   Expression

   Bacterial

   Mutation

   Codon for Tyr47 replaced with amber stop codon TA…

   Promoter

   hsp60 and metU

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-FLEX-rc[Chronos-GFP]

   Plasmid

   #59056

   Purpose

   Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCLXSN(GFP)-HA-Slc1a5 (mouse)

   Plasmid

   #71458

   Purpose

   inducible expression fo Slc1a5 in mammalian cells

   Depositor

   Shao-Cong Sun

   Article

   Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

   Insert

   Slc1a5 (Slc1a5 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   A85P, L125F and V384A mutations compared to GenBa…

   Promoter

   CMV

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pHIV-EGFP.Flag-EZH2-mt2*

    Plasmid

    #220246

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2* (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PRKKKR494-499NAAIRS

    Promoter

    EF-1α

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HsNot1iso1_1093-1317_W

    Plasmid

    #147892

    Purpose

    Mammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot1iso1_1093-1317 (CNOT1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn-CsChR-GFP

    Plasmid

    #58841

    Purpose

    AAV expression of CsChR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CsChR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV-hGFAP-GFP-T2A-SOX2

    Plasmid

    #183908

    Purpose

    Expression of GFP and human SOX2 under the human GFAP ABC1D promoter

    Depositor

    Chun-Li Zhang

    Article

    Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

    Insert

    SOX2 (GFAP, SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-APLP1ICD-IRES-hrGFP

    Plasmid

    #107546

    Purpose

    AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    APLP1ICD (APLP1 Human)
    

    Use

    AAV

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.RMacL3-01.ChR2(H134R)-GFP.BCC10

    Plasmid

    #229639

    Purpose

    L3 pyramidal neuron Enhancer to drive transgene expression

    Depositor

    William Stauffer

    Article

    He et al Neuron. 2025 May 21;113(10):1548-1561.e8

    Insert

    RMacL3-01.ChR2(H134R)-GFP.BCC10
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Qt-FLAG-TGA-rt9us-antiGFP

    Plasmid

    #206954

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Expression

    Mammalian

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Qt-FLAG-TAA-rt20s-antiGFP

    Plasmid

    #206955

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1M-Qt-FLAG-TGA-rt20s-antiGFP

    Plasmid

    #206953

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHW394 (15xUAS::GFP::let-858 3'UTR)

    Plasmid

    #85584

    Purpose

    cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    15xUAS-delta pes-10-GFP
    

    Expression

    Worm

    Mutation

    GFP(S65C) with three introns for expression in C.…

    Promoter

    15xUAS-delta pes-10-GFP

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid

    #84563

    Purpose

    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    

    Use

    Lentiviral

    Tags

    myc tag (9x)

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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