We narrowed to 3,597 results for: Gla
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pET30a-ASXL1-HARE-HTH domain
Plasmid#107164PurposeExpresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coliDepositorInsertASXL1 (Asxl1 Mouse)
TagsFlagExpressionBacterialMutationContains amino acids 2-83PromoterT7Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
B0032m_BC
Plasmid#66020PurposeMoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]DepositorInsertRBS
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT1-1 (Q867E)-intein-CBD
Plasmid#73301PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationQ867EAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_EF
Plasmid#66064PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP1
Plasmid#160084PurposeBacterial expression of Tn5-APEX2 fusion protein, no linkerDepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP2
Plasmid#160085PurposeBacterial expression of Tn5-APEX2 fusion protein with linker PAPAPDepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM mGata3
Plasmid#25796DepositorInsertGata3 (Gata3 Mouse)
ExpressionBacterialAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm:Rm_AF
Plasmid#66061PurposeMoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm:Gm_AF
Plasmid#66062PurposeMoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXG-hXRCC1-K176R
Plasmid#52284PurposeBacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein and GSTDepositorInsertXRCC1 (XRCC1 Human)
TagsGyrA-intein-GSTExpressionBacterialMutationK176R, SUMO acceptor site mutantPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT3-1 (C935N)-intein-CBD
Plasmid#73304PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationC935NAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTGFR-DuET
Plasmid#213371PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEndo-I-OnuI-TSM
Plasmid#207954PurposeExpresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.DepositorInsertThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
UseSynthetic BiologyExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in fu…Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM 1996
Plasmid#217825PurposeExpresses putative photoreceptor SA-phr1 from S. alba. This gene shows high homology to the phr genes in prokaryotes.DepositorInsertSA-phr1
TagsMaltose-binding proteinExpressionBacterialAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGER1-DuET
Plasmid#213370PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
N229A Lo-MetQ
Plasmid#118268PurposeN229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structureDepositorInsertMetQ (N229A) (metQ E. coli)
Tags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationChanged Asparagine (Asn) 229 to Alanine (Ala)PromoterT7 PromoterAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD
Plasmid#73303PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationE1083QAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD
Plasmid#73299PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationS1079N-E1083QAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_EF
Plasmid#66060PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only
