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pUC-UAS1B5-Leum

Plasmid

#44313

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B5-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B5-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B10-Leum

Plasmid

#44318

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B10-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B10-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p413TEF-Sir2-P394L

Plasmid

#51744

Purpose

overexpression of Sir2 in S. cerevisiae (P394L mutant), HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Mutation

P394L

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(203)

Plasmid

#44378

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(203)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(804)

Plasmid

#44368

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(804) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(804)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pRB7-6

Plasmid

#24700

Depositor

George Allen

Article

Allen et al Plant Cell. 1996 May . 8(5):899-913.

Insert

Tobacco Rb7 MAR (SAR)

Use

E. coli phagemid

Available Since

July 8, 2010

Availability

Academic Institutions and Nonprofits only
pNDGG015

Plasmid

#231336

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-p15A-Gm-ELT3; pNDGG021, pOGG009, pNDGG006, pOGG013. NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFABs9

Plasmid

#241905

Purpose

Ferrulic acid biosensor - expresses sfGFP in response to substituted cinnamic acid

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

sfGFP, ferC, ferA

Use

Synthetic Biology

Mutation

none

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p131C-B10

Plasmid

#241908

Purpose

Optimised PCA biosensor produced sfGFP in response to hydroxyl-substituted benzoic acids, including protocatechuic acid (PCA)

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

pcaV, sfGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 Rab23

Plasmid

#237337

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

Rab23 (RAB23 Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-RYBP NZF (20-51)

Plasmid

#235652

Purpose

Bacterial expression of human RYBP NZF (20-51)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

RYBP NZF (RYBP Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 20-51 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG014

Plasmid

#231335

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG003

Plasmid

#231330

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site for BpiI cloning; KmR/NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-TO-g0 (FLP-IN)

Plasmid

#236120

Purpose

Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

g0

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW434 4x(ZNF35/ZNF35tar)-SFFV-GFP

Plasmid

#236143

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/ZNF35 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/ZNF35 binding sites…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW435 4x(ZNF35/IKZF1tar)-SFFV-GFP

Plasmid

#236144

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/IKZF1 binding sites…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW420 4x(ZNF35/ZNF250tarv3)-SFFV-GFP

Plasmid

#236139

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/ZNF250 binding site…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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L1p6_barley_sgRNA_scaffold

Plasmid

#231033

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

Depositor

Wilma van Esse

Article

Winkelmolen et al bioRxiv 2024.08.22.609225

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p5_barley_sgRNA_scaffold

Plasmid

#231032

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

Depositor

Wilma van Esse

Article

Winkelmolen et al bioRxiv 2024.08.22.609225

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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