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Addgene

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We narrowed to 8,294 results for: construct

Showing: 3341 - 3360 of 8294 results

  13. pLN34 - pBait-5GC[1xMS2]-McaS

    Plasmid
    #222403
    Purpose
    arabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.
    Depositor
    Katherine Berry
    Article
    Linh D. Nguyen et al Sci Rep 15, 3820 (2025)
    Insert
    McaS (mcaS E. coli)
    Use
    Tags
    1xMS2 hairpin, 5GC clamp
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    222403-plasmid-map-sequence-id-451849

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  16. SUMO_LPETG_MT3

    Plasmid
    #200304
    Purpose
    Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200304-plasmid-map-sequence-id-394896

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  17. SUMO_MT3

    Plasmid
    #200303
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200303-plasmid-map-sequence-id-394864

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  19. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193314
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193314-plasmid-map-sequence-id-382366

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  20. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

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Showing: 3341 - 3360 of 8294 results