We narrowed to 6,447 results for: nls

Showing: 3341 - 3360 of 6447 results

Results per page:

Sort results by:

Tet1-dCas9

Plasmid

#136650

Purpose

Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

Tet1 (Tet1 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Tet1-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_TRE3GS-->GssGFP-P2A-NLSIFP

Plasmid

#162235

Purpose

Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

GFP - IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol1-uas-mAKT-2a-nlstdTomato

Plasmid

#67706

Purpose

Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

mAKT-2A-nlstdTomato

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pattB-nsyb-MKIIK11A::nlsLexADBDo

Plasmid

#64726

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKIIK11A::nlsLexADBDo

Expression

Insect

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mPA-GFP-PCNA-19-SV40NLS-4

Plasmid

#57154

Purpose

Localization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
RPL25NLS-GFP-PRA (102)

Plasmid

#24041

Depositor

Michael Rout

Insert

RPL25 (RPL25 Budding Yeast)

Tags

EGFP and PRA

Expression

Yeast

Mutation

Only bp 1-135 (aa 1-45) of RPL25

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pY095

Plasmid

#84744

Purpose

Expresses huLbCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
GFP

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Nme2Cas9_pCDest

Plasmid

#119923

Purpose

Nme2Cas9 CMV-driven mammalian expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Tags

NLS-3xHA-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHygR8

Plasmid

#200221

Purpose

hygR selection nls-mScarlet marked rapid RMCE integration vector without loxP site

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHygG8

Plasmid

#200220

Purpose

hygR selection nls-mNG marked rapid RMCE integration vector without loxP site

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA2675

Plasmid

#189997

Purpose

Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

Depositor

Bungo Akiyoshi

Article

Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

Inserts

FBP75
VhhGFP4

Use

Expression vector for trypanosoma brucei

Tags

NLS

Mutation

N-terminal 600 bp of FBP75

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CIB-VP16 (B1014)

Plasmid

#92036

Purpose

Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Fusion of plant CIB1 (intact NLS) with VP16 activ…

Expression

Mammalian

Promoter

T7

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_MYC deltaNLS_P2A_Hygro_Barcode

Plasmid

#120506

Purpose

Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC H84NLS (MYC Human)

Use

Lentiviral

Mutation

Deletion of nuclear localization signal sequence

Promoter

EF1a

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
3xnls-G-Ca-FLITS

Plasmid

#191463

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

3xNLS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge