We narrowed to 5,829 results for: pcas

1. pYPQAT04

   Plasmid

   #223376

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSK-Spsg

   Plasmid

   #214351

   Purpose

   Expresses cloning backbone for SpCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 tracrRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3919-32

   Plasmid

   #176496

   Purpose

   pCAG-puro-T2A-TetON3G-polyA

   Depositor

   Albert La Spada

   Article

   Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

   Insert

   puroR-T2A-TetOn3G
   

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA197

   Plasmid

   #149602

   Purpose

   (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFP

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   yeGFP
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   PcaO_1O8

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL1211

   Plasmid

   #129521

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFlag-NLS

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH70

   Plasmid

   #128190

   Purpose

   Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR0SP0014

   Plasmid

   #117524

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-KA

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-KA (with stop codon)
   

   Use

   CRISPR

   Mutation

   K855A

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR0SP0009

   Plasmid

   #117522

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-p (no stop codon)
   

   Use

   CRISPR

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICSL90005

   Plasmid

   #117520

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-h (no stop codon)
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. sABE v3.22_C

    Plasmid

    #209554

    Purpose

    pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0052-REPAIR non-targeting guide clone into pC0043

    Plasmid

    #103868

    Purpose

    Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    non-targeting guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBWB419

    Plasmid

    #140637

    Purpose

    RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Expression

    Bacterial

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Expression

    Yeast

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin promoter

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCRi

    Plasmid

    #207524

    Purpose

    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    

    Use

    Pseudomonas vector

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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