We narrowed to 3,642 results for: ORF1

Showing: 3361 - 3380 of 3642 results

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pAG416GALL-SARS-CoV_3CL

Plasmid

#203495

Purpose

Expresses SARS-CoV 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-Bat-CoV-HKU4_3CL

Plasmid

#201943

Purpose

Expresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-CoV HKU4 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-NL63_3CL

Plasmid

#201938

Purpose

Expresses HCoV-NL63 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-NL63 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCoV-NL63_PLP2

Plasmid

#203502

Purpose

Expresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-NL63 PLP2 (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCoV-OC43_3CL

Plasmid

#203503

Purpose

Expresses HCoV-OC43 3CL protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-OC43 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-ΔN-CHMP4B(S184C)

Plasmid

#180026

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

S184C

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

Plasmid

#198765

Purpose

expression of hOCT4 mutants

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 236D (POU5F1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S236D

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-WFIKKN1-COMP5AP-AviTag-9xHis

Plasmid

#157469

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

WFIKKN1 (WFIKKN1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp7 (SARS-CoV-2)

Plasmid

#169178

Purpose

Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7 (ORF1ab Synthetic, SARS-CoV-2)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1c_nsp8 (SARS-CoV-2)

Plasmid

#169179

Purpose

Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp8 (ORF1ab Synthetic, SARS-CoV-2)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-TOM20MTS-DDRGK1-dTM-HA

Plasmid

#139861

Purpose

Lentiviral expression of TOM20MTS-DDRGK1-dTM-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

TOM20-MTS-DDRGK1-dTM (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

recombinant fusion of mitochondria-targeting sign…

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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IDNK gRNA (BRDN0001146514)

Plasmid

#75865

Purpose

3rd generation lentiviral gRNA plasmid targeting human IDNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IDNK (C9orf103 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IDNK gRNA (BRDN0001147773)

Plasmid

#75866

Purpose

3rd generation lentiviral gRNA plasmid targeting human IDNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IDNK (C9orf103 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IDNK gRNA (BRDN0001148428)

Plasmid

#75867

Purpose

3rd generation lentiviral gRNA plasmid targeting human IDNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IDNK (C9orf103 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

Plasmid

#89992

Purpose

Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)

Use

CRISPR

Promoter

none

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

Plasmid

#214137

Purpose

Insect cell expression of the human 20SCP proteasome assembly chaperones

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

POMP (POMP Human)
PSMG1 (PSMG1 Human)
PSMG2 (PSMG2 Human)
PSMG3 (PSMG3 Human)
PSMG4 (PSMG4 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA4D-COMP5AP-AviTag-9xHis

Plasmid

#157516

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA4D (SEMA4D Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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