We narrowed to 20,261 results for: ACE;

1. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AsdCas12a VPR

   Plasmid

   #188512

   Purpose

   Expresses FLAG tagged AsdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-basic-hRARβP-FL-luciferase

   Plasmid

   #135447

   Purpose

   Human full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vector

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Full-length promoter of human retinoic acid receptor-beta gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Human RARβ promoter

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. XLone-BSD SPI1

   Plasmid

   #179514

   Purpose

   Tunable and temporal expression control of SPI1

   Depositor

   Xiaoping Bao

   Article

   Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS Promoter

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.GFP-AP1/gamma1

   Plasmid

   #218801

   Purpose

   Mammalian expression of mouse Ap1 g1

   Depositor

   Marvin Bentley

   Article

   Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

   Insert

   Ap1 g1 (Ap1g1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Affibody HER2:243.BirA*-pET301/NT-DEST

   Plasmid

   #174692

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2_UBE2:mT-N7-CsA

   Plasmid

   #136133

   Purpose

   Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35S:HygR p5-MpUBE2:mTurq-N7
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. MTK2_005

   Plasmid

   #123700

   Purpose

   Encodes pCAG as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pCAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. phGR-SB

   Plasmid

   #133409

   Purpose

   overexpression of a human glucocorticoid receptor in mammalian cells

   Depositor

   Andrew Cato

   Article

   Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

   Insert

   NR3C1 (NR3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Golgi-ExRai-AktAR2

    Plasmid

    #184051

    Purpose

    Golgi-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Golgi-ExRai-AktAR2
    

    Use

    Tags

    N-terminal 35 amino acids from human endothelial …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGREAT2

    Plasmid

    #170890

    Purpose

    GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    p35SoTMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Use

    Tags

    yomRuby3

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-MR1

    Plasmid

    #178603

    Purpose

    Mammalian expression of myc-tagged MR1

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Use

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGREAT1

    Plasmid

    #170889

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FMRP shRNA

    Plasmid

    #206074

    Purpose

    expression of FMRP RNA for gene silencing in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Ferron et al Nat Commun. 2014 Apr 7;5:3628. doi: 10.1

    Insert

    FMRP (Fmr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. opto-Itsn1

    Plasmid

    #174509

    Purpose

    Expresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Itsn1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK3_036

    Plasmid

    #123750

    Purpose

    Encodes mScarlet as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-4591NES

    Plasmid

    #138377

    Purpose

    Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-ROCK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJBL7056

    Plasmid

    #167225

    Purpose

    orthogonal T7 RNAP sfGFP reporter

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pAKSIRV-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RnBarr2-mCherry

    Plasmid

    #137794

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGGS-4580NES

    Plasmid

    #138376

    Purpose

    Booster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-JNK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACDura3

    Plasmid

    #138711

    Purpose

    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Insert

    ura3 gene (URA3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    optimized ura3 gene for expression in Escherichia…

    Promoter

    pEM7 promoter

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

    Plasmid

    #217681

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB1 (ADGRB1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-926

    Promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Use

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EYFP Cav2.2 pRK5

    Plasmid

    #206099

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTU2-A-RFP (p15A origin)

    Plasmid

    #74090

    Purpose

    pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    R0010 Const. Promoter controlling RFP

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. opto-Tiam1

    Plasmid

    #174508

    Purpose

    Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Tiam1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330-PITCh-MYO1C

    Plasmid

    #227305

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge