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Addgene

We narrowed to 7,509 results for: PAC

Showing: 6721 - 6760 of 7509 results
  1. pdCas9-GFP-p65

    Plasmid
    #232630
    Purpose
    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer
    Depositor
    Insert
    dCas9-GFP-p65 (RELA Synthetic, Human)
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. LVDP 2P.2.EGFP

    Plasmid
    #231905
    Purpose
    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPs
    Depositor
    Insert
    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    R203M in N protein
    Promoter
    CMV, EF-1a
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pDIV299

    Plasmid
    #204967
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker
    Depositor
    Inserts
    Mad7
    argB
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. CROPseq-Puro-12xMS2

    Plasmid
    #212628
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Insert
    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid
    #99696
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pYPQ121-Split-TALED

    Plasmid
    #218171
    Purpose
    Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein
    Depositor
    Insert
    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
    Use
    Plastid base editing
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pCitro-15a

    Plasmid
    #202033
    Purpose
    p15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter
    Depositor
    Insert
    conjugative system from a Citrobacter spp.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. E1_pENTR_L1-L4_NI_G1333-DddtoxA-C

    Plasmid
    #171724
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. E1_pENTR_L1-L4_NN_G1333-DddtoxA-C

    Plasmid
    #171728
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. E3_pENTR_L3-L2_NG_G1333-DddtoxA-C

    Plasmid
    #171732
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. E1_pENTR_L1-L4_NI_G1333-DddtoxA-N

    Plasmid
    #171725
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. E1_pENTR_L1-L4_NN_G1333-DddtoxA-N

    Plasmid
    #171729
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. E3_pENTR_L3-L2_NG_G1333-DddtoxA-N

    Plasmid
    #171733
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

    Plasmid
    #166986
    Purpose
    Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
    Depositor
    Insert
    Termination module:PolyU(1), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

    Plasmid
    #166991
    Purpose
    Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
    Depositor
    Insert
    Termination module:PolyU(4), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

    Plasmid
    #166990
    Purpose
    Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
    Depositor
    Insert
    Termination module:PolyU(1), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

    Plasmid
    #166987
    Purpose
    Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
    Depositor
    Insert
    Termination module:PolyU(4), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 6721 - 6760 of 7509 results