We narrowed to 7,278 results for: 11

1. MTK7_013

   Plasmid

   #123880

   Purpose

   Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   RA_hAAVS1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK3_008

   Plasmid

   #123723

   Purpose

   Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   NLS-VP16-EL222
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EBFP2-Cx43

   Plasmid

   #69020

   Purpose

   Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Tags

   EBFP2

   Promoter

   CMV

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNEXdelta-EGFP-ApAF

   Plasmid

   #13335

   Depositor

   Bong-Kiun Kaang

   Article

   Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

   Insert

   Aplysia activating factor
   

   Tags

   EGFP

   Available Since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

5. MTK2_004

   Plasmid

   #123699

   Purpose

   Encodes mouse pPGK1 as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pPGK1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEasyG3_hph

   Plasmid

   #184919

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIneo-V5-SBP-HseIF4AII_AC

   Plasmid

   #148439

   Purpose

   Mammalian Expression of HseIF4AII

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HseIF4AII (EIF4A2 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK4a_019

   Plasmid

   #123832

   Purpose

   Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   d2PEST tag
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW102

   Plasmid

   #115945

   Purpose

   ORF insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 DDd-L7Ae
   

   Use

   Synthetic Biology

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnEA-NpH-HsNot9_S

    Plasmid

    #147494

    Purpose

    Bacterial Expression of HsNot9

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

    Insert

    HsNot9 (CNOT9 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-EF1alpha-HA-TFAP4

    Plasmid

    #110158

    Purpose

    Lentiviral vector for expression of human TFAP4 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK0_014

    Plasmid

    #123929

    Purpose

    Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBIattB destination vector
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK8b_005

    Plasmid

    #123893

    Purpose

    Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' AAV ITR
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK3b_021

    Plasmid

    #123802

    Purpose

    Encodes VPR activation domain as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    VPR AD
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK3a_026

    Plasmid

    #123778

    Purpose

    Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sadCAS9 (with NLS)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid

    #140201

    Purpose

    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Cloning of crrna an…

    Tags

    dLbCas12a crRNA scaffold

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. JBEI-3716

    Plasmid

    #100169

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    codon-optimized MK
    

    Expression

    Bacterial

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-synPCB2.0

    Plasmid

    #139477

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Expression

    Mammalian

    Mutation

    V413L

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB3020(gRNA X-2)

    Plasmid

    #73282

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. JPF0510

    Plasmid

    #124001

    Purpose

    Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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