1,862 results

1. pSA110

   Plasmid

   #59784

   Purpose

   drives mCherry in C. elegans intestinal cells

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   elt-2 promoter (elt-2 Nematode)
   mCherry (worm)
   unc-54 3' UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCFJ355 - MCS(ttTi14024, X)

   Plasmid

   #34870

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   ttTi14024 targeting
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDD288

   Plasmid

   #137017

   Purpose

   mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   mNG-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized mNG

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEY26

   Plasmid

   #191031

   Purpose

   cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   cho-1(3.3-2.6k) (cho-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEY39

   Plasmid

   #191043

   Purpose

   acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-2(2.1k) (acr-2 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TC476

   Plasmid

   #122629

   Purpose

   Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJA304

   Plasmid

   #28091

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTK73.ChrII_ttTi5605.sgRNA

   Plasmid

   #194058

   Purpose

   sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrII_ttTi5605 sgRNA
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTK73.ChrV_oxTi365.gRNA

   Plasmid

   #194052

   Purpose

   sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrV_oxTi365 sgRNA
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEY52

    Plasmid

    #191055

    Purpose

    odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    odr-1 (odr-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSDH61

    Plasmid

    #139033

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (no STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBT3037

    Plasmid

    #122544

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Synthetic, Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDSP32

    Plasmid

    #199374

    Purpose

    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pNM3732

    Plasmid

    #153086

    Purpose

    mec-4p GFP-C1 positive control for RMCE integration

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p GFP-C1 tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JH03

    Plasmid

    #73512

    Purpose

    mKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mKate2::PH::tbb-2
    

    Use

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    mex-5

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCM5.37

    Plasmid

    #17253

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    unc-54 3' from pPD49.78 in pDONRP2RP3
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ210

    Plasmid

    #30538

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BSP188

    Plasmid

    #110917

    Purpose

    For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    unc54 Terminator
    

    Use

    Synthetic Biology; Yeast homologous recombination…

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWP018

    Plasmid

    #198766

    Purpose

    Drives specific expression in the ADE, CEP, and PDE neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pdat-1-GAL4-SK(DBD)-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW2072

    Plasmid

    #154334

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge