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Showing: 641 - 680 of 1186 results

  2. pPLP1H-mCf

    Plasmid
    #229984
    Purpose
    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229984-plasmid-map-sequence-id-458288

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  3. pPLP2H-mCf

    Plasmid
    #229985
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229985-plasmid-map-sequence-id-458287

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  4. pPLP3H-mCf

    Plasmid
    #229987
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229987-plasmid-map-sequence-id-458283

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  6. pPLP4HS-mCf

    Plasmid
    #229988
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    His6 and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229988-plasmid-map-sequence-id-458286

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  7. pPLP2S-mCf

    Plasmid
    #229986
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    Strep-tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229986-plasmid-map-sequence-id-458306

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  8. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92065-plasmid-map-sequence-id-181210

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  9. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

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  10. phage - ubc - nls - mcp - egfp - BirA*

    Plasmid
    #131134
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-MCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131134-plasmid-map-sequence-id-255499

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  11. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131136-plasmid-map-sequence-id-255502

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  12. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154787-plasmid-map-sequence-id-306329

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  13. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131133-plasmid-map-sequence-id-255501

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  14. pHR-tdMCP-YFP

    Plasmid
    #99151
    Purpose
    Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector
    Depositor
    Ron Vale
    Article
    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do
    Insert
    Dimeric MS2-coat binding protein
    Use
    Lentiviral
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    99151-plasmid-map-sequence-id-190042

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  16. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  21. pMH205

    Plasmid
    #122861
    Purpose
    Binary expression vector containing pSUC2:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modules
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    pSUC2:MS2-VP64:tRBCS
    Use
    Golden gate compatible plant transformation vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122861-plasmid-map-sequence-id-239404

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  22. pMH186

    Plasmid
    #122837
    Purpose
    MS2-NLS (no stop codon) with GreenGate C-D flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-NLS, codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Tags
    Nuclear localisation signal
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122837-plasmid-map-sequence-id-239367

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  27. pJK2014

    Plasmid
    #171931
    Purpose
    To express sygl-1 fused to 24 copies of MS2 loops.
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
    Use
    Tags
    N-terminal Tag 24X MS2 loops
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171931-plasmid-map-sequence-id-337176

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  30. pMH183

    Plasmid
    #122856
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122856-plasmid-map-sequence-id-247655

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  31. pMH184

    Plasmid
    #122857
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122857-plasmid-map-sequence-id-239400

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  32. Lenti-SAM-Puro

    Plasmid
    #198713
    Purpose
    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198713-plasmid-map-sequence-id-402626

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  33. Lenti-SAM-Cre

    Plasmid
    #198714
    Purpose
    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pgk and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198714-plasmid-map-sequence-id-402628

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  37. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Use
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  39. pTK332

    Plasmid
    #59573
    Purpose
    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59573-plasmid-map-sequence-id-92978

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Showing: 641 - 680 of 1186 results