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We narrowed to 2,760 results for: FAS

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Showing: 801 - 850 of 2760 results

  3. pX018

    Plasmid
    #167144
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal luciferase, nnLuz
    Use
    Luciferase and Synthetic Biology
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167144-plasmid-map-sequence-id-335106

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  6. pX019

    Plasmid
    #167145
    Purpose
    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal hispidin-3-hydroxylase, nnH3H
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167145-plasmid-map-sequence-id-335228

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  7. pET21-10XHis-GST-HRV-dL5-Her1

    Plasmid
    #73215
    Purpose
    Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    EGFR (EGFR Human, Synthetic, Schistosoma japonicum)
    Tags
    10XHis-GST-HRV and The dL5 FAP and Her1 affibody …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73215-plasmid-map-sequence-id-137064

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  29. pX030

    Plasmid
    #167154
    Purpose
    MoClo-compatible Level 1 (position 5) vector encoding Neonothopanus nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-nnCPH-ocsT
    Expression
    Plant
    Promoter
    p35s
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167154-plasmid-map-sequence-id-335275

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  31. pX022

    Plasmid
    #167148
    Purpose
    MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    C-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167148-plasmid-map-sequence-id-335231

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  32. pX021

    Plasmid
    #167147
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    N-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167147-plasmid-map-sequence-id-335230

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  35. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid
    #162876
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    GFP Binding Protein tagged with mEos2
    Tags
    mEos2
    Expression
    Mammalian
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162876-plasmid-map-sequence-id-321989

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  36. pHybr_r2a>mA-srt-his

    Plasmid
    #124806
    Purpose
    HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgA
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124806-plasmid-map-sequence-id-244320

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  37. CIRTS-control

    Plasmid
    #213788
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213788-plasmid-map-sequence-id-424349

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  38. CIRTS-Gas5

    Plasmid
    #213172
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-Gas5 gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213172-plasmid-map-sequence-id-424348

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  39. pHybr_r2a>mG2b-srt-his

    Plasmid
    #124804
    Purpose
    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2b
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124804-plasmid-map-sequence-id-244314

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  40. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

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  41. pHybr_r2a>mG1-srt-his

    Plasmid
    #124802
    Purpose
    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG1
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Promoter
    -
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124802-plasmid-map-sequence-id-244317

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  42. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  43. pHybr_r2a>mG3-srt-his

    Plasmid
    #124805
    Purpose
    HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG3
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124805-plasmid-map-sequence-id-244310

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  46. pBlue-hFX(I154F)-HA

    Plasmid
    #14988
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed isoleucine 154 to phenylalanine
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  47. pBlue-hFX(T133A)-HA

    Plasmid
    #14989
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed threonine 133 to alanine
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  48. pBlue-hFX(T133E)-HA

    Plasmid
    #14990
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed threonine 133 to glutamic acid
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  49. pBlue-hFX-HA(AscI)

    Plasmid
    #15404
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    700 bp insert can be released by digestion with r…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15404-plasmid-map-sequence-id-275749

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  50. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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Showing: 801 - 850 of 2760 results