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pEPSW1KN0035

Plasmid

#196177

Purpose

MoClo Level 1 luciferase reporter: NOS::TMV::LucN::flag::nosT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

NOS::TMV::LucN::flag::nosT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0018

Plasmid

#196176

Purpose

MoClo Level 1 luciferase reporter: proARF18::LucN::flag::notT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

proARF18::LucN::flag::notT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0020

Plasmid

#196175

Purpose

MoClo Level 1 luciferase reporter: proNLP6::LucN::flag::notT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

proNLP6::LucN::flag::notT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0014

Plasmid

#196174

Purpose

MoClo Level 1 luciferase reporter: proANAC032::LucF::flag::notT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

proANAC032::LucF::flag::notT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1868

Plasmid

#170417

Purpose

Genetic complementation plasmid of LetAB with LetB mutant L599N

Depositor

Damian Ekiert, Gira Bhabha

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB E. coli)

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(L599N)

Promoter

T7

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCk2chlo

Plasmid

#163943

Purpose

Acceptor plasmid for odd level position 2

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk3chlo

Plasmid

#163944

Purpose

Acceptor plasmid for odd level position 3

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk4chlo

Plasmid

#163945

Purpose

Acceptor plasmid for odd level position 4

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pC0.065

Plasmid

#119659

Purpose

Level 0 part. Terminator araBAD operon from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010860

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.068

Plasmid

#119658

Purpose

Level 0 part. Terminator for clpPX gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010850

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mCherry-MinD-IRES-Puro

Plasmid

#213112

Purpose

Expression of mCherry-MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE-EGFP_IRES-Blast

Plasmid

#213113

Purpose

Expression of MinE-EGFP in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE-EGFP (minE Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTJV1Sc3-rpoB

Plasmid

#166983

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

RecT (recT E. coli)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pVvanCC

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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OLIG2_pCSdest

Plasmid

#53858

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

OLIG2 (OLIG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-MinC

Plasmid

#133628

Purpose

Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

Depositor

Petra Schwille

Article

Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

Insert

His-MinC (minC E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

Plasmid

#214459

Purpose

Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinD-IRES-Puro

Plasmid

#213114

Purpose

Expression of untagged MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinD (minD Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKD317.8

Plasmid

#181951

Purpose

IPTG-inducible expression of NarX

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

NarX-Ptac; lacI-PlacIq

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

James Williamson, T. J. Kappock

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MYMH 464

Plasmid

#138068

Purpose

Natural Terminator ECK120033736 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-33736-GFP-pSEVA-331Bb

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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