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Showing: 321 - 340 of 354 results

  1. Zebrafish aBb crystallin 2kb promoter/AcGFP

    Plasmid
    #98099
    Purpose
    Zebrafish aBb crystallin 2kb promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98099-plasmid-map-sequence-id-193847

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  3. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid
    #123181
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123181-plasmid-map-sequence-id-241286

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  5. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid
    #113685
    Purpose
    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    inverted de-catalyzed SaCas9
    Use
    AAV, CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    NLS and VP64
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113685-plasmid-map-sequence-id-232027

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  6. Mouse aB crystallin 1.5kb promoter/AcGFP

    Plasmid
    #98104
    Purpose
    Mouse aB crystallin 1.5kb promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha B-crystallin promoter 1.5 kb fragment, Mus musculus (Cryab Mouse)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Alpha B-crystallin 1.5 kb promoter fragment (-150…
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98104-plasmid-map-sequence-id-193852

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  7. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid
    #123180
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123180-plasmid-map-sequence-id-241283

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  8. pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS

    Plasmid
    #123200
    Purpose
    binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123200-plasmid-map-sequence-id-242251

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  9. Mouse aB crystallin 250bp promoter/AcGFP

    Plasmid
    #98102
    Purpose
    Mouse aB crystallin 250bp promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Alpha B-crystallin promoter 250 bp fragment (-259…
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98102-plasmid-map-sequence-id-193850

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  10. Mouse aB crystallin 800bp promoter/AcGFP

    Plasmid
    #98103
    Purpose
    Mouse aB crystallin 800bp promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Alpha B-crystallin promoter 800 bp fragment (-833…
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98103-plasmid-map-sequence-id-193851

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  13. pSB61 - pL0_V2 (CDS1)

    Plasmid
    #123183
    Purpose
    Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
    Use
    Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123183-plasmid-map-sequence-id-241416

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  14. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid
    #123182
    Purpose
    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    Use
    Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123182-plasmid-map-sequence-id-241413

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  15. pOpen-UDG

    Plasmid
    #165531
    Purpose
    Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    UDG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165531-plasmid-map-sequence-id-339117

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  16. pOpen-SP6rpol

    Plasmid
    #165516
    Purpose
    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SP6 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165516-plasmid-map-sequence-id-338647

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  17. pOpen-T7EI

    Plasmid
    #165540
    Purpose
    DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T7EI (T7 Endonuclease I)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165540-plasmid-map-sequence-id-339262

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  18. pOpen-T3gene1

    Plasmid
    #165576
    Purpose
    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T3 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165576-plasmid-map-sequence-id-340988

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  19. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165551-plasmid-map-sequence-id-339868

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Showing: 321 - 340 of 354 results