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Plasmid#100797Purposeexpression of shRNA targeting SRFDepositorInsertrat SRF
UseRNAiTagsExpressionMutationPromoterH1Available sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-soCoChR-GFP
Plasmid#107711PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterCaMKIIAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-Nix
Plasmid#100770PurposeLentiviral expression of shRNA targeting NixDepositorInsertLenti-sh-Nix
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-soCoChR-GFP
Plasmid#107710PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterCAGAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-soCoChR-mCardinal
Plasmid#107713PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoterDepositorInsertsoCoChR-mCardinal
UseAAVTagsKA2 and mCardinalExpressionMammalianMutationPromoterhSynAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-GFP
Plasmid#107709PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMVc-Cas9
Plasmid#106431PurposeExpresses SpCas9 from the CMVc promoter in AAV backboneDepositorInsertCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoterCMVcAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-nEF-dCas9
Plasmid#106430PurposeExpresses SpdCas9 from the nEF promoter in AAV backboneDepositorInsertnEF-dCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoternEFAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGmCherry-gRNA
Plasmid#87110PurposegRNA cloning vector with CAGmCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-JEDI-2P
Plasmid#179461PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expressionDepositorInsertJEDI-2P
UseTagsExpressionInsectMutationPromoterhsp70Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA (pLP140)
Plasmid#168461PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA2 (pLP300)
Plasmid#168462PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA3 (pLP305)
Plasmid#196992PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tubb3gRNA-mCherry
Plasmid#87111Purposemouse Tubb3 target gRNA expression plasmid with CAGmCherryDepositorInsertMouse Tubb3 gRNA
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEFCas9
Plasmid#87115PurposeSpCas9 expression AAV backbone plasmid under control of nEF promoterDepositorInsertnEF-Cas9
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-WPRE
Plasmid#179460PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorInsertMS2-P65-HSF1 (HSF1 Synthetic, Human)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-2P-WPRE
Plasmid#179464PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSynDepositorHas ServiceAAV9InsertJEDI-2P
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-JEDI-2P-Kv-WPRE
Plasmid#179465PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertJEDI-2P-Kv
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only