We narrowed to 2,123 results for: PAM

1. pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5

   Plasmid

   #226352

   Purpose

   Expression of Titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1

   Plasmid

   #226353

   Purpose

   Expression of titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-Dopamine D2 receptor [N186/26R]

   Plasmid

   #222156

   Purpose

   Mammalian Expression Plasmid of anti-Dopamine D2 receptor (Rat). Derived from hybridoma N186/26.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Dopamine D2 receptor (Rattus norvegicus) recombinant mouse monoclonal antibody. (Drd2 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT314 AAV-Rapamycin Inducible-NZ-Citrine

   Plasmid

   #202063

   Purpose

   Inducible AAV activator

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-NZ-Citrine
   

   Use

   AAV; Aav with reporter

   Expression

   Mammalian

   Mutation

   ITR deletion

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT365 AAV-Rapamycin Inducible-S3H

   Plasmid

   #202066

   Purpose

   Inducible AAV activator

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-S3H
   

   Use

   AAV; Aav with reporter

   Expression

   Mammalian

   Mutation

   ITR deletion

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT366 AAV-Rapamycin Inducible QNZF

   Plasmid

   #202067

   Purpose

   Inducible AAV activator

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible QNZF
   

   Use

   AAV; Aav with reporter

   Expression

   Mammalian

   Mutation

   ITR deletion

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT313 AAV-Rapamycin Inducible-ZF-Citrine

   Plasmid

   #202062

   Purpose

   Inducible AAV activator

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-ZF-Citrine
   

   Use

   AAV; Aav with reporter

   Expression

   Mammalian

   Mutation

   ITR deletion

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

   Plasmid

   #208645

   Purpose

   Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation for knock-in of…

   Tags

   Twin Strep

   Mutation

   Two SNP (rs749555789, rs760194863) were introduce…

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmGW182-PAM26B-dsRNAres_L

   Plasmid

   #146837

   Purpose

   Insect Expression of DmGW182-PAM26B-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   DmGW182-PAM26B-dsRNAres (gw Fly)
   

   Expression

   Insect

   Mutation

   Two non silent mutations V704A and N799S and one …

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCaggs-NLS-PAmCherry1-GSS-EGFP

    Plasmid

    #158000

    Purpose

    Expression of nucleus localized PAmCherry1 and EGFP fusion protein

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    NLS-PAmCherry1-GSS-EGFP
    

    Expression

    Mammalian

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM-PAT-ProCDKA;1::CDKA;1:YFP

    Plasmid

    #80685

    Purpose

    binary Expression vector for plant expression

    Depositor

    Nico Dissmeyer, Arp Schnittger

    Article

    Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

    Insert

    CDKA;1 (CDC2 Mustard Weed)
    

    Tags

    YFP

    Expression

    Plant

    Promoter

    ProCDKA;1

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

    Plasmid

    #203770

    Purpose

    For production of template RNA for use in PRINT, mCherry under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for mCherry
    

    Use

    Ivt template

    Promoter

    CMV

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TCARZ-CMV-GFP-PAmin-GF3-R4A22

    Plasmid

    #203768

    Purpose

    For production of template RNA for use in PRINT, GFP under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Promoter

    CMV

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TCARZ-SV40-GFP-PAmin-GF3-R4A22

    Plasmid

    #203769

    Purpose

    For production of template RNA for use in PRINT, GFP under SV40 promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Promoter

    SV40

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

    Plasmid

    #159630

    Purpose

    AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

    Depositor

    Ross Bathgate, Andrew Allen

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    Ast (AstA Fly)
    

    Use

    AAV

    Tags

    HA, IRES, mCherry, and neurophysin-1

    Expression

    Mammalian

    Promoter

    DCA (same as CAG promoter)

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT311 AAV-Rapamycin Inducible-NZF-Citrine

    Plasmid

    #202060

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NZF-Citrine
    

    Use

    AAV; Aav with reporter

    Expression

    Mammalian

    Mutation

    ITR deletion

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid

    #215769

    Purpose

    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

    Depositor

    Dimitrios Wagner

    Article

    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

    Inserts

    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Human, Bovine, Mouse)
    TRAC-3' homology arm (TRAC Human)
    

    Expression

    Bacterial

    Mutation

    Cas12a PAM mutated

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

    Plasmid

    #177320

    Purpose

    Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

    Depositor

    Steven Chu, Xiaolin Nan

    Article

    Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Insert

    KRAS 4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP-derived dimerization domain (DD, or DmrB) an…

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    TetOn-CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-CMV-EGFP-PolB-PAMmut-Hygro

    Plasmid

    #176086

    Purpose

    EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by PolBKOg1

    Promoter

    CMV

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-EF1a-CDK6-mutPAM-PGK-puro

    Plasmid

    #187638

    Purpose

    sg resistant CDK6 ORF

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    PAM site mutation

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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