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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,254 results for: gfp lenti

Showing: 321 - 340 of 3254 results

  2. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid
    #215014
    Purpose
    Express an autophagy (LC3) reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    MAP1LC3B (Map1lc3b Rat)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215014-plasmid-map-sequence-id-429267

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  7. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

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  9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  13. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118163-plasmid-map-sequence-id-240400

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  16. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

    Plasmid
    #202717
    Purpose
    Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Inserts
    Myr-Pink Flamindo
    EGFP-bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    202717-plasmid-map-sequence-id-402853

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  17. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118159-plasmid-map-sequence-id-240446

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  18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118164
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118164-plasmid-map-sequence-id-240433

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  19. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118160
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118160-plasmid-map-sequence-id-240351

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Showing: 321 - 340 of 3254 results