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We narrowed to 470 results for: plasmid cloning by pcr

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Showing: 321 - 340 of 470 results

  1. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

    Plasmid
    #40767
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed plus 13-16 target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  9. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

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  10. pFS556

    Plasmid
    #242856
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetS promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetS promoter
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242856-plasmid-map-sequence-id-486278

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  11. pFS558

    Plasmid
    #242858
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW2 promoter
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242858-plasmid-map-sequence-id-485663

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  12. pFS559

    Plasmid
    #242859
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW3 promoter
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242859-plasmid-map-sequence-id-485659

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  13. pFS560

    Plasmid
    #242860
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW4 promoter
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242860-plasmid-map-sequence-id-485654

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  14. pFS557

    Plasmid
    #242857
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW1 promoter
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242857-plasmid-map-sequence-id-484506

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  15. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140980-plasmid-map-sequence-id-300680

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Showing: 321 - 340 of 470 results