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Hy_pMT Laccase2 Sense

Plasmid

#69883

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMT Draper-GFP

Plasmid

#126653

Purpose

Inducible expression of Draper-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT Crk-mCherry

Plasmid

#126659

Purpose

Inducible expression of Crk-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT vCrk-GFP

Plasmid

#126661

Purpose

Inducible expression of vCrk truncation in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Use

Tags

Expression

Insect

Mutation

residues 1-182

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Use

Tags

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT Shark-mCherry

Plasmid

#126668

Purpose

Inducible expression of Shark-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT-sox10-cytoBirA-2A-mCherry_Ras

Plasmid

#80062

Purpose

Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Sox10 promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-T2A-Venus(L68V)

Plasmid

#60494

Purpose

Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2-T2A-mVenus(L68V)

Use

Tags

T2A peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-PMT-miR-34a

Plasmid

#25799

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-34a promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
cacnb2a (rat) in pMT2 vector

Plasmid

#107424

Purpose

Expresses Calcium channel beta2a auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

Insert

calcium channel beta2a auxillary subunit (Cacnb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pL1M-pMtRC-MtLAP1-Adh-R1

Plasmid

#170790

Purpose

A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

Depositor

Jeremy Murray

Article

Ruan et al Plants (Basel). 2021 Mar 23;10(3). pii:

Insert

MtLAP1 (LOC11412013 Medicago truncatula)

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Medtr4g059670(MtRC)

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-E488Q-V5

Plasmid

#139685

Purpose

Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Hrp48, ADAR (Adar Fly)

Use

Tags

V5

Expression

Bacterial and Insect

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Use

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Mutation

Promoter

pMT

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 HA pMT2

Plasmid

#58729

Purpose

expression of HA tagged rat Alpha2 delta-1 in mamalian cells

Depositor

Annette Dolphin

Article

Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(CmYLCV)

Plasmid

#202014

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Use

Tags

GB1 and sfGFP

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Use

Tags

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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cacnb4 (rat) in pMT2 vector

Plasmid

#107426

Purpose

Expresses Calcium channel beta4 auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

Insert

cacnb4 (Cacnb4 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-2 pMT2

Plasmid

#58732

Purpose

expression of mouse Alpha2 delta-2 in mammalian cells

Depositor

Annette Dolphin

Article

Barclay et al J Neurosci. 2001 Aug 15;21(16):6095-104.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-2 HA pMT2

Plasmid

#58731

Purpose

expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

Depositor

Annette Dolphin

Article

Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-3 pMT2

Plasmid

#58727

Purpose

expression of mouse Alpha2 delta-3 in mammalian cells

Depositor

Annette Dolphin

Article

Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

Insert

alpha2 delta-3 (Cacna2d3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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