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pBM16

Plasmid

#45344

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata expression

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGRB2.1

Plasmid

#45341

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGRB2.0

Plasmid

#45340

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBM16.1

Plasmid

#45345

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-flag-YTHDF2-N

Plasmid

#52302

Purpose

Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Mutation

Changed E384 to stop codon

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-flag-YTHDF2-C

Plasmid

#52303

Purpose

Flag-tagged YTHDF2 C-terminal domain, from E384 to the end

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Mutation

Deleted amino acids 1-383

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (830-839)

Plasmid

#188091

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (830-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (692-839)

Plasmid

#188087

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-Foldon-ATG9 (801-839)

Plasmid

#188089

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-Foldon-ATG9 (723-839)

Plasmid

#188088

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (723-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-Foldon-ATG9 (828-839)

Plasmid

#188090

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (828-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-OSF-ATG13 (2-197)-K18D

Plasmid

#188096

Purpose

Expresses human ATG13 HORMA K18D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-K18D

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-K15D

Plasmid

#188094

Purpose

Expresses human ATG13 HORMA K15D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-W50D

Plasmid

#188098

Purpose

Expresses human ATG13 HORMA W50D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-W50D

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-Y152D

Plasmid

#188103

Purpose

Expresses human ATG13 HORMA Y152D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-Y152D

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-OSF-ATG13 (2-197)-R153D

Plasmid

#188104

Purpose

Expresses human ATG13 HORMA R153D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-R153D

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-OSF-ATG13 (2-197)-F16D

Plasmid

#188095

Purpose

Expresses human ATG13 HORMA F16D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-F16D

Promoter

CMV

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-Y118D

Plasmid

#188101

Purpose

Expresses human ATG13 HORMA Y118D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-Y118D

Promoter

CMV

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge