We narrowed to 917 results for: tac promoter

1. poly H35S SUMO1

   Plasmid

   #221071

   Purpose

   Expression of poly H35S SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   H35S

   Promoter

   tac

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. poly E67R SUMO1

   Plasmid

   #221072

   Purpose

   Expression of poly E67R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   E67R

   Promoter

   tac

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. poly H35K SUMO1

   Plasmid

   #221070

   Purpose

   Expression of poly H35K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   H35K

   Promoter

   tac

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. poly H35R SUMO1

   Plasmid

   #221069

   Purpose

   Expression of poly H35R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   H35R

   Promoter

   tac

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIB184-Km

   Plasmid

   #90195

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

   Depositor

   Indranil Biswas

   Article

   Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EW369 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)

   Plasmid

   #236145

   Purpose

   Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSC24

   Plasmid

   #104798

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101120, Medtr2g101130
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC19

   Plasmid

   #104793

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g020630
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_G2_Dual_sgRNA

   Plasmid

   #173201

   Purpose

   Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

    Plasmid

    #178110

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid

    #173214

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF

    Plasmid

    #236153

    Purpose

    Express mutated SCN1A-14 zinc finger array (G2E, A104K, S109I) attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    SCN1A-377-deImmLink-NZF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G2E, A104K, S109I in SCN1A-377

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA

    Plasmid

    #178108

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA

    Plasmid

    #178109

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMKO.1-shNuf2

    Plasmid

    #160961

    Purpose

    Nuf2 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nuf2 shRNA (Nuf2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. EW492 CMV-TO-UTRN-14ZF(N61K,H88F, I91E, K120F)-realdeImmLink-NZF(FLP-IN)

    Plasmid

    #236150

    Purpose

    Express mutated UTRN-14 zinc finger array (N62K, H89F, I91E, K121F) attached by a deimmunized linker to the NZF transcriptional activation under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    UTRN-14ZF-deImmLink-NZF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N61K, H88F, I91E, K120F in UTRN-14ZF

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVD437

    Plasmid

    #63758

    Purpose

    7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)

    Depositor

    James Kaper

    Article

    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

    Insert

    eae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. VV229: GCaMP6f in fck

    Plasmid

    #58514

    Purpose

    Expresses GCaMP6f under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Tags

    C-terminal FCYENEV (ER export motif); this was no…

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. VV201: ChR2(C128S)-eGFP in fubi

    Plasmid

    #58510

    Purpose

    Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    ChR2(C128S)-eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Cysteine 128 to Serine

    Promoter

    Ubiquitin

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. VV220: CoChR(C108S)-eGFP in fck

    Plasmid

    #58512

    Purpose

    Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    CoChR(C108S)-eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 108 to Serine

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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